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題名 | 從豬隻分離所得巴斯德桿菌之型別鑑定與試製抗原對家兔及豬隻之免疫 |
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作者 | 陳清; 澤田拓士; | 書刊名 | 臺灣畜牧獸醫學會會報 |
卷期 | 65:3 1995.09[民84.09] |
頁次 | 頁239-245 |
分類號 | 437.245 |
關鍵詞 | 巴斯德桿菌; 間接血球凝集反應; 瓊脂免疫沈降反應; 血清型; 豬; Pasteurella multocida; Serotype; Indirect hemagglutination test; Swine; Agar gel precipitation test; |
語文 | 中文(Chinese) |
中文摘要 | 由供試豬鼻腔內病材分離所得之9株巴氏桿菌,使用間接血球凝集反應(Indirect hemagglutination test, IHA test)及瓊脂免疫沈降反應(Agar gel precipitation test, AGP test)作型別及菌體抗原鑑定之結果,得知Type A有5株,Type D有4株,而菌體抗原則圾為第3型。以不同型別菌株,試製抗原免疫家兔及豬隻之結果,家兔之IHA抗體價,因型別及免疫個體之不同而有所差異,自1:32至1:2,048不等,而免疫豬所產生之抗體價偏低,但經攻擊而耐過者則呈明顯上昇。 |
英文摘要 | Indirect hemagglutination (IHA ) test and agar gel precipitation (AGP) test was used to for serotyping and somatic antigen identification of nine strains of Pasteurella multocida isolated from nasal cavities of swine. Five strains of type A capsule antigen and four of type D were identified. All the somatic antigens are observed to belong to serotype 3. Immunogens prepared from various serotype of Pasteurella multocida strains were used to immunize rabbits and swine. IHA antibody titer of immunized rabbits ranged from 1:32 to 1:2,048 depending on the types and strains. The antibody titer for immunize swine were low. However, the IHA titer increased significantly in resistant swine after challenge. |
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