查詢結果分析
來源資料
相關文獻
- Expression of Recombinant Human Interferon Gamma in Escherichia Coli
- Expression of a Bioactive Murine Interleukin-18 Protein
- Production of Recombinant Oreochromis Mossambicus Growth Hormone (GH) Polypeptides in E. Coli Cells and Characterization of the Molecular Structure of the GH Gene
- Differential Expression of Cytokine Genes and Apoptosis in Glioma Cell Lines upon Exposure to Bacteria and Lipopolysaccharides
- Influence of Cefodizime on the Proliferative Response and the Cytokine Production by Human Mononuclear Cells
- 鼻咽癌中D1細胞素之表現
- Naloxone Suppresses Cytokines Production in LPS-stimulated Human Monocytic THP-1 Cells
- Lowered IL-4-producing T Cells and Decreased IL-4 Secretion in Peripheral Blood from Subjects with Juvenile Rheumatoid Arthritis
- Influence of Gamma Irradiation and Storage on Apheresis Platelets
- 探討氣喘病人在接受中藥治療前後體內Th1/Th2比例和細胞素製造及血中發炎物質濃度差異
頁籤選單縮合
題 名 | Expression of Recombinant Human Interferon Gamma in Escherichia Coli=利用大腸桿菌(Escherichia coli)表現重組型人類丙型干擾素 |
---|---|
作 者 | 邱繡河; 蔡寧遠; 毛嘉洪; 何素鵬; | 書刊名 | 臺灣畜牧獸醫學會會報 |
卷 期 | 69:1/2 民88.06 |
頁 次 | 頁1-10 |
分類號 | 437.24 |
關鍵詞 | 丙型干擾素; 細胞素; 蛋白質表現; Interferon gamma; Cytokine; Protein expression; |
語 文 | 英文(English) |
中文摘要 | 利用大腸桿菌(Escherichia coli)表現重組型人類丙型干擾素丙型干擾素為一種 廣效的細胞素,在調控免疫與發炎反應方面扮演重要角色。為了生產丙型干擾素蛋白質以供 獸醫相關研究及臨床應用,本研究選殖成熟的人類丙型干擾素之重組型基因,分別轉接至原 核表現載體 pTrc99A 及 pET32a 以構築人類丙型干擾素與其融合蛋白之表現載體 pTrc-hIFN 及 pET-hIFN。 利用細菌轉型技術分別將此兩載體送入大腸桿菌 (E.coli) 菌株 JM105 及 BL21(DE3)pLysS,經異丙基硫化蒎喃型半乳糖甘 (IPTG) 誘導後, 分別產生與所 預期分子量相符的 17kDa 重組型丙型干擾素及 34kDa 丙型干擾素融合蛋白。 |
英文摘要 | Interferon gamma (IFN-γ) is a pidotropic cytokine that has important roles in regulating immune and inflammatory processes. To produce IFN- γ protein for veterinary research and clinical application, the engineered gene for mature human interferon gamma was cloned into prokaryotic expression vectors pTrc99A and pET32a to construct human IFN- γ and its fusion protein expression vectors, pTrc-hIFN γ and pET-hIFN γ, respectively. By bacterial transformations, both expression vectors were introduced separately into Escherichia coli (E.coli) strain JM105 and strain BL21(DE3) pLysS. After induction with isopropyl- β -D-thiogalactopyranoside (IPTG), bacterial strains carrying pTrc-hIFN γ and pET-hIFNy produced recombinant IFN- γ and IFN- γ fusion protein, with an expected molecular weight of 17 kDa and 34 kDa, respectively. |
本系統中英文摘要資訊取自各篇刊載內容。