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| 題 名 | 假性狂犬病毒(臺灣TNL株)封套醣蛋白gp50基因之結構分析 |
|---|---|
| 作 者 | 鍾楊聰; 汪良儒; 張天傑; | 書刊名 | 臺灣畜牧獸醫學會會報 |
| 卷 期 | 65:2 1995.06[民84.06] |
| 頁 次 | 頁119-130 |
| 分類號 | 437.246 |
| 關鍵詞 | 假性狂犬病毒; 基因選殖; 結構分析; Pseudorabies virus; Gene cloning; Structure analysis; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 假性狂犬病病毒(pseudorabies virus)是引起豬隻感染假性狂犬病(pseudorabies,又稱Aujeszky's disease)之致病原。此病毒封套至少含有六種醣蛋白(gⅠ、gⅡ、gⅢ、gp50、g63、gH),其中gp50與病毒之毒力展現有關,已被證實可引發中和抗體之產生。本實驗係利用遺傳工程技術,自台灣南部分離得到之神經型強毒性假性狂犬病毒TNL株中分離出完整的gp50基因,並加以核甘酸定序。吾人共決定1494個核甘酸序列,其中包含了完整的gp50基因轉譯區(coding region)1206個鹼基對。此基因序列的A+T含量為26%,G+C含量為74%,經與美國Rice株gp50基因序列相比對,有高達99.2%的相同率。此外在gp50基因轉譯區上游可見類及TATA box的保留性序列(consensus sequence),但下游區則未見有可辨識的ploy(A)訊息序竟(polyadenylation signal sequence)。根據核甘酸序列所推導出來的蛋白質gp50的安基酸序列及結構,顯示其具備膜蛋白與O-鍵結型醣蛋白的基本特徵。 |
| 英文摘要 | Pseudorabies virus (PRV) is a herpesvirus of swine that causes significant disease, particularly in young pigs. It has been known that the viral envelope contains at least six glyco-proteins, i.e., gⅠ, gⅡ, gⅢ, gp50, gp63, and gH. Of these proteins, gp50 is thought to function in attachment and penetration of virus into susceptible cells and be important in the induction of cellular immunity to PRV. Using recombinant DNA techniques, we have cloned the gp50gene from the PRV TNL strain previously isolated in southern Taiwan and completed its nucleotide sequence analysis. A 1,206 nucleotide long sequence was identified as the gp50 open reading frame (ORF). The nucleotide composition of the ORF was calculated to be 26% A+T and 74% G+C. Comparison of this gp50 ORF with that previously reported for the PRV Rice strain revealed very high homology in these two sequences. Except for eight single base substitutions and one single base insertion, our results indicate that the TNL strain and Rice strain share the same gp50 sequence. Moreover, the deduced amino acid sequence shows its lack of potential site for N-linked glycosylation, supporting the notion that gp50 is an unusual O-linked glycoprotein. |
本系統中英文摘要資訊取自各篇刊載內容。