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題 名 | 以酵素免疫法及高效能液相層析法檢驗飼料中Terbutaline=Determination of Terbutaline in Feeds Using Enzyme-linked Immunosorbent Assay and High Performance Liquid Chromatography |
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作 者 | 戴東發; 華宏達; 卓世賢; 劉朝鑫; | 書刊名 | 臺灣畜牧獸醫學會會報 |
卷 期 | 68:3/4 民87.12 |
頁 次 | 頁149-154 |
分類號 | 437.111 |
關鍵詞 | 酵素免疫法; 高效能液相層析法; 飼料; ELISA; HPLC; β-agonists; Terbutaline; |
語 文 | 中文(Chinese) |
中文摘要 | 以0.O1N HC1萃取飼料中之terbutaline後,利用RANDOX公司出品ELISA kit檢測 作初步篩選檢驗樣品中心β -agonists 之初步篩選檢驗,其最低檢測值為 1 ppb。 有陽性 反應之樣品,再做 terbutaline 條件之 HPLC 確認測試。 terbutaline 之檢測為重新取樣 以 0.01N HC1 萃取, 以 1N NaOH 調整 pH,再以 C18 column 吸附後以甲醇沖提,收集液 經 HPLC 分離以螢光檢測器定量。檢測條件為激發波長:275nm, 放射波長:305nm。最低確認 檢測感度為 0.1ppm; 回收率為 65-72%。 |
英文摘要 | Methods were developed for the extraction, Enzyme Immunoassay ( EIA ) an HPLC for terbutaline. The procedure of immunoassay involved the extraction of terbutaline in feeds with O.O1N HCI, centrifugation and then the supernatant was examined by the EIA. The lowest detection limit was 1 ppb. If the sample was found positive by EIA, repeated extraction was carried out with 0.0 IN HCl, passed through the C18 column elution, and the presence of terbutaline was confirmed with HPLC fluorescence detector at excitation wavelength of 275nm and emission wavelength of 305nm. The detection limit of terbutaline by HPLC was 0.1 ppm, and the range of the recovery rate was 65-72% |
本系統中英文摘要資訊取自各篇刊載內容。