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頁籤選單縮合
題名 | 水稻懸浮細胞對逆境之反應 |
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作者姓名(中文) | 曾梅慧; 童武夫; | 書刊名 | 師大生物學報 |
卷期 | 30:1 1995.10[民84.10] |
頁次 | 頁31-40 |
分類號 | 434.114 |
關鍵詞 | 水稻細胞; 逆境; 酶活性誘導; Rice cells; Stress; Enzyme induction; |
語文 | 中文(Chinese) |
中文摘要 | 本研究是以逆境處理來探討水稻細胞在逆境下的生長及生理反應。逆境處理包括在培養基中添加20mMNaCl、1.63μMHP2及真菌抽出物,也使用去除含磷成份之培養基。逆境處理都普遍會抑制細胞生長並降低細胞內可溶性蛋白質含量,但在影響程度上有差別。從蛋白質的電泳分析,可以觀察到在缺磷條件下,雖然蛋白質總量大幅降低,仍有新的蛋白質(26Kd)的出現,而且分子量59Kd蛋白質含量也會增高,甚至釋出細胞外的35Kd蛋白質也有明顯增加。鹽逆境下細胞的生長並無明顯受到抑制,但促使細胞內超氧歧化酶、觸媒酶、過氧化氫酶活性的增高,並促進酸性磷酸酶的分泌泣。真菌抽出物也一樣能促使超氧歧化酶及過氧化氫酶活性增高,但不影響酸性磷酸酶活性。缺磷逆境下細胞生長受到抑制,細胞內可溶性蛋白質也明顯降低,然而酸性磷酸酶活性無論是細胞外或細胞內卻大幅增高;從異構酶分析中發現有新異構酶的出現,然而反映酶活性的增高應是細胞內的API及細胞外的APOl異構酶。這些結果顯示細胞會籍生理代謝的改變來適應不同逆境,以求取生存。 |
英文摘要 | Cell growth and physiological responses of rice suspension cells (Oryza sativa cv. Tainan 5) in the phosphorous deficient medium or exposed to 20 mM NaCl, 1.63μM Hydrogen peroxide or fungal elicitor were investigated. Cell growth and soluble protein content in cell were inhibited under the stresses. Changes of soluble protein pattern in the media and the cell extract were analyzed by SDS-PAGE. A 59 Kd protein was increased and a 26 Kd protein was induced with phosphate deficient treatment. 26 Kd protein seems to be a specific protein appeared only with phosphate deficiency. The results were further confirmed with transferred experiments. Cell growth was not obviously affected with salt treatment, while the activity increase of superoxide dismutllse, catalase and peroxidase in cells as well as extracellular acid phosphatase were observed. Although serious inhibition of both cell growth and protein synthesis were shown under the stress of phosphorous deficiency, activities of intra- and extracellular acid phosphatase increased with the time of stress treatment. Isozyme patterns of acid phosphatase in cells were changed with the stress treatment, but the main isozymes API and APO1 should be responsible for the activity increase. The results indicate that stress could affect the cells to terminate some proteins to be synthesized and to synthesize certain new proteins simultaneously. For adaptation to the stressful environment cells should inevitably adjust the metabolism of itself in order to survive. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。