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題 名 | 赤桉的癒合組織培養及再生之研究 |
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作 者 | 張淑華; 楊政川; | 書刊名 | 林業試驗所研究報告季刊 |
卷 期 | 10:1 1995.03[民84.03] |
頁 次 | 頁15-24 |
分類號 | 436.259 |
關鍵詞 | 赤桉; 癒合組織培養; 芽體再生; Eucalyptus camaldulensis; Callus culture; Shoot regeneration; |
語 文 | 中文(Chinese) |
中文摘要 | 取在台灣表現最佳之來自澳洲Petford赤桉種源的種子苗下胚軸與子葉培養於B5、MS或WPM培養基中添加30g/l蔗糖、100mg/l casein hydrolysate、200mg/l glutamine、100ml/l coconut milk (分別簡稱 B5C、MSC或WPMC)、0-1mg/l cytokinins (BA、kinetin)及3-5 mg/l auxins (NAA、2,4-D)。培養1週後癒合組織會在下胚軸或子葉的切口及表面產生,其顏色有黃、綠、白和紅色。在培養30天時,癒合組織生長達到高峰,若繼續培養於相同培養基超過45天不給予繼代培養,則癒合組織會褐化死亡。下胚軸及子葉癒合組織的再生,均以培養在B5C培養基較MSC及WPMC中的為佳,且兩者癒合組織再生芽體,都只發生在含NAA的培養基,2,4-D對芽體再生沒有效果。最適合兩者芽體再生之最佳培養基為B5C培養基添加3mg/l NAA及1mg/l BA。除了培養基組成外,光線也是癒合組織芽體再生必要的條件。芽體的再生形態有三種:(1)直接由培植體上長出、(2)由培植體先分化出葉原體或(3)分化出癒合組織再長出芽體。癒合組織的芽體分化可由鬆散、緊密或瘤狀的細胞團產生,但可長期保持再生力的只有瘤狀的癒合組織,其他的組織在經繼代培養2-3次後就喪失再生能力。赤桉的瘤狀癒合組織經繼代培養超過8個月,仍可維持再生能力。切下癒合組織再生的芽體來培養,很容易在培養基中發根,生長成健壯的小苗。 |
英文摘要 | In vitro seedlings of Eucalyptus camaldulensis were raised from the surface sterilized seeds introduced from Petford, Australia which performed the best in provenance test in Taiwan. Hypocotyl and cotyledon explants excised from tnese seedlings were cultured on B5, MS or WPM medium containing 30 g/l sucrose, 100 mg/l casein hydrolysate, 200 mg/l glutamine, 100 ml/l coconut milk (coded as B5C, MSC or WPMC, irrespectively), 0 to 1 mg/l cytokinins (BA or kinetin) and 3 to 5 mg/l auxins (NAA or 2,4-D). The callus occurred on the cut edges and the surface of cotyledon and hypocotyl explants within a week and the color of callus was initially yellow, green, white and red, then turned brown if kept them on the same medium for more than 45 days. Calli derived from hypocotyl and cotyledon regenerated more shoots on B5C medium than on MSC and WPMC media. Shoots occurred only on NAA medium but not on 2,4-D medium. The optimal medium to form shoots for these two explants was B5C medium containing 3 mg/l NAA and 1 mg/l BA in light. Shoot regeneration was induced in three ways: some shoots were directly from explant tissues, some were initiated from primordial leaf tissues which derived from explants, most were from callus. Structures of callus that formed shoots were friable, conpact and module tissues, however, only nodule tissues maintained regenerability in long term subculturing. The other two tissues lost regnerability after subculturing twice or thrice. Shoots derived from calli rooted readily and developed into healthy plantlets. |
本系統中英文摘要資訊取自各篇刊載內容。