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| 題 名 | 第一型類胰島素生長因子(IGF-I)對腦轉移肺癌細胞具自主分泌刺激效應 |
|---|---|
| 作 者 | 黃秋琴; 方剛; 施河; | 書刊名 | 師大生物學報 |
| 卷 期 | 29:2 1994.12[民83.12] |
| 頁 次 | 頁57-64 |
| 分類號 | 415.132 |
| 關鍵詞 | 類胰島素生長因子; 自主分泌刺激效應; 癌細胞轉移; Insulin-like growth factor; Autocrine; Metastasis; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 第一型類胰島素生長因子(IGF-I)對細胞之生長發育有重要的調節作用。在許多癌細胞株中,IGF-I 也扮演自主分泌刺激(autocrine)的作用。為分析IGF-I在腫瘤形成過程中的變異情形,本文就非細小肺癌細胞株(NSCLC)及其腦移轉細胞株,探討IGF-I及其受體(IGF-I)之基因表現情形。由反轉錄酶帶動熱聚合連鎖反應(RT-PCR)實驗顯示所用的兩種原生非小肺癌細胞株(包括巨大細胞株H460及鱗細胞株H226)僅有極少量IGF-I表現,但肺癌腦轉移細胞株(H226B)則表現出大量的IGF-I mRNA。在受體方面,所有測試細胞株均有相近之IGF-I-R mRNA表現。而以IGF-I-R單株抗體(αIR3)作細胞增殖試驗顯示,H226B之生長會受到抗體增加而有抑制遞增現象,而其原生細胞H226受IGF-I-R抗體影響則不明顯。這些結果顯示出肺癌腦轉移細胞株會分泌IGF-I以調節自身的生長,而在轉移過程後細胞必需依賴以IGF-I主導之自主分泌刺激效應機制。 |
| 英文摘要 | Insulin-like growth factor I (IGF-I) is important in regulating cell growth and development. IGF-I fimctions as an autocrine growth stimulus in many cancer cell lines. In order to analyze the fimction of IGF-I in tumor progression, IGF-I, its receptor gene expression and peptide secretion were examined in non-small-cell lung cancer cell lines (NSCLC) and the brain metastatic variant. Reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that, compared to two cultured NSCLC cell lines (H226 and H460), the brain metastatic variant H226B showed IGF-I gene expression elevation. All cell lines examined expressed same levels of IGF-I-R gene as demonstrated by RT-PCR. It was also found that αIR3, an IGF-I-R specific antibody, inhibited the growth of H226B. These results suggest that IGF-I is critical in the regulation of cell growth of H226B.The growth of lung tumor brain metastasis progression is regulated by IGF-I-mediated autocrine mechanism. |
本系統中英文摘要資訊取自各篇刊載內容。