頁籤選單縮合
題 名 | 檳榔生物鹼對人類口腔纖維母細胞生長之影響 |
---|---|
作 者 | 黃正芬; 陳慶長; 蔡吉政; | 書刊名 | 嘉南學報 |
卷 期 | 20 1994.11[民83.11] |
頁 次 | 頁123-137 |
分類號 | 416.94 |
關鍵詞 | 檳榔; 纖維母細胞; Betel nut; Arecoline; Arecaidine; Fibroblast; |
語 文 | 中文(Chinese) |
中文摘要 | 嚼食檳榔是國人相當普遍的一種習性,而且有日益嚴重的趨勢。根據 文獻指出,嚼食檳榔對口腔組織包括牙齒、牙齦及口腔粘膜均有明顯不良的影 響,進而可能引發癌前期病變(如:白斑症、粘膜下纖維化症)與口腔癌。但直至目 前,有關檳榔成分引起口腔病變之真正機轉仍不清楚。本研究之目的是以細胞培 養的方法,來分析檳榔生物鹼一Arecoilne與Arecaidine對口腔頰粘膜與牙齦纖維 母細胞生長之影響,進而探討檳榔生物鹼在口腔病變發生上所可能扮演的角色。 實驗中,分別採用MTT比色法與BrdU標定法測量細胞增殖與DNA合成情形。 結果顯示:(1)頰粘膜與牙齦纖維母細胞之形態相似,且兩種細胞對AreColine與 Arecaidine之反應形式相近。(2)Arecoline與Arecaidine有刺激纖維母細胞增殖與 DNA合成之作用,但當Arecoline濃度為100ug/ml時,則具有細胞毒性。(3)各細 胞株之間,對ArecOline或Arecaidine感受性仍有些微差異。由以上結果。我們推 測檳榔生物鹼-Arecolint與Arecaidine對口腔纖維母細胞之刺激生長與細胞毒性作 用,可能與口腔病變之發生有密切關係。至於其確切角色.則仍有待進一步的研 究。 |
英文摘要 | Betel quid chewing habits are commonly seen in the people of Taiwan and the prevalence ofbetel quid chewing is increasing gradually. Many investigators have suggested that betel quid chewing has a untoward effect on the tissues of the oral cavity, including teeth, gingiva and oral mucosa. Furthermore, it has been reported that, compared with those who do not chew betel quid atall, the risk of oral precancerous lesions (e. g. leukoplakia and oral submucous fibrosis) and oralcancer are higher among those who chew betel quid; however, the relative risk associated witheach ingredient used in the quid has not been established clearly. In the present study, we investigated the effects of betel nut alkaloids (arecoline and arecaidine) on the cell proliferation andDNA synthesis of oral fibroblasts which were obtained from healthy oral buccal mucosa and healthy gingiva. The results could be summarized as follows: (l)There was no difference in cellularmorphology of fibroblasts from the buccal mucosa and gingival tissues, and the patterns of cellular response to arecoline and arecaidine were similar between the two kinds of oral fibroblasts.(2)With the exception for arecoline which was cytotoxic at 100 ug/ml, we found that arecolineand arecaidine can stimulate cell proliferation and DNA synthesis of the oral fibroblasts. (3)Therewere some variations in the response to arecoline or arecaidine among all of the cell lines. Suchfindings imply that there are stimulations of cell growth of human oral fibroblasts in vitro by betel nut alkaloids. However, the exact role between the oral lesions and betel nut alkaloids needsto be investigated further. |
本系統中英文摘要資訊取自各篇刊載內容。