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題名 | 自配酒精酵素試劑定量酒精方法之評估= |
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作者 | 林碧芬; 賴滄海; |
期刊 | 慈濟醫學 |
出版日期 | 19940900 |
卷期 | 6:3 1994.09[民83.09] |
頁次 | 頁171-178 |
分類號 | 412.37 |
語文 | chi |
關鍵詞 | 酒精; 自動化; 酵素法; Ethanol; Automation; Enzymatic assay; |
中文摘要 | 本研究以日立747臨床化學分析儀,及ELISA Reader和96-well Microplate,配合自製的試劑,使用酒精去氫酵素(alcohol dchydrogenase.ADH)進行 酒精濃度的檢驗。並與市面上所售之Sigma Kits進行評估比較,評估項目包括速 度、成本、儀器設備、精密度、準確度、干擾等條件,以提供檢驗室或實驗室參 考選擇使用。研究結果顯示三者精密度皆很高,CV<4,除了Microplate方法的CV 範圍較大,在低濃度時CV=8.6。以日立747和Sigma Kits進行分析時,基質不會影 響酒精濃度,但以Microplate法分析.則不同基質會造成判讀誤差。如用同類基 質之正常檢體當空白值,可消除不同基質所造成之誤差。ADH酵素法會受 n-butanol和isopropanol醇類影響,以t-test分析,P值遠小於0.005,而葡萄糖的加 入並沒有顯著影響(P>0.05)。至於三個方法之間的相關性,由迴歸統計得知彼此 皆有很高的相關(r>0.96)。綜合而言此三法在精密度、準確度是沒有明顯差異, 所以我們可針對經費、儀器、實驗需求,交互使用這些方法,而不影響酒精濃度 的判讀。(慈濟醫學1994:6:171-178) |
英文摘要 | The reagent for the enzymatic assay of ethanol was prepared and adapted to automated clinical chemistryanalyzer (Hitachi 747) and 96-well microplate with automated ELISA Reader. These two methods were compared with the Sigma commercial kits. The precision of these three methods is very high, and the percentages ofcoefficient of variance (CV ) are all less than 4 except at the low ethanol concentration by the microplatemethod. There is no matrix effect by the Hitachi 747 method and the Sigma kits, and the matrix effect of themicroplate method could be eliminated by using a normal sample of the same matrix as a blank. As reported byothers, n-butanol and isopropanol interfere with the assay, but the addition of glucose (5 mg/ml) has no effect.The three methods correlate well with each other (r �� > 0.96). In conclusion, these three methods could be usedexchangably, and the concentration of the measured ethanol is not affected. (Tzu-Chi Med J 1994; 6:171-178) |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。