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頁籤選單縮合
題名 | 應用母長頸鹿糞中孕酮濃度變化於其生殖狀況之探討 |
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作者 | 李素蘭; 張希賢; 陳玉燕; 趙明杰; 楊健仁; 陳寶忠; 林仁壽; 吳兩新; | 書刊名 | 動物園學報 |
卷期 | 6 1994.03[民83.03] |
頁次 | 頁53-60 |
分類號 | 389.887 |
關鍵詞 | 長頸鹿; 糞孕酮; 懷孕; 動情週期; 酵素免疫分析法; Giraffe; Estrous cycle; Pregnancy; Enzyme immunoassay; Fecal progesterone; |
語文 | 中文(Chinese) |
中文摘要 | 本試驗應用糞便中孕酮濃度的測定,探討臺北市立動物園雌性長頸鹿(Giraffa camelopardalis)的生殖狀況,以作為繁殖管理的參考。在15頭雌性長頸鹿中,包括7頭成 熟母鹿及8頭未成熟雌鹿。自1991年9月起,3頭母鹿(#5~7)及8頭未成熟雌鹿,每 2~3天採取糞樣1次,歷時2個月;另外4頭母鹿(#1~4),除懷孕及泌乳時,每週採 樣一次外,其他時間每2~3天採樣1次,母鹿#l及#3於1992年12月,而#2及4於1993 年3月中旬停止採樣。糞樣經甲醇和石油醚萃取後,應用微滴盤酵素免疫分析法(EIA) 測定其孕酮含量。試驗結果顯示:長頸鹿的動情週期為16天(n=2),黃體期之糞孕酮濃 度為1000~5000ng╱g,而濾泡期為300ng╱g以下。懷孕期長度為470天(n=1),糞孕酮 濃度為6062±2784ng╱g(n=5)。分娩後,其泌乳乏情期為3~7個月,而未哺乳者約在1 個月後即恢復動情週期。未發身的8頭雌鹿,有2頭之糞孕酮 濃度顯著高於成熟母鹿之濾 泡期濃度;而其餘6頭之糞孕酮濃度則很低。在臺北市立動物園中的長頸鹿各項生殖資料, 均與其他作者的報告相符合,顯示糞孕酮濃度的測定的確能清楚的反映其動情週期的變化 以及懷孕等生殖狀態。 |
英文摘要 | The object of this study was to investigate the reproduction of the giraffe (Giraffa camelopardalis) by means of measuring fecal progesterone. Fifteen female giraffes (7 parous and 8 nulliparous) reared in Taipei Zoo were used. Their stools were collected periodiclly between 2-7 days for about 2 months to 2 years depending on the experimental designs and frozen at-20℃ until assayed. The fecal progesterone were extracted and determined by an enzyme immunoassay. There was a 15-17 day estrous cycle (N=2 animals) with a 3-6 day follicular phase proven by the changes of progesterone patterns in which the fecal progesterone levels were below 300 ng/g in follicular phase and between 1000-5000 ng/g in mid-luteal phase. An intact gestation period with 470 days observed in one animal and her fecal progesterone concentrations with other 4 femals averaged 6062±2784 ng/g during the pregnacy. After parturition, a marked decline in fecal progesterone excretion was evident. The estrus return around 1 month for the non-lactating giraffe and 3-7 months for the lactating ones after parturition was also confirmed by rapid raise in fecal progesterone excretion. Generally, the fecal proges-terone levels of unpubertal giraffes were kept below 300 ng/g as those of mature ones at follicular phase. However, 2 out of 8 nulliparous females showed some higher fecal progesterone values kept around those of mature ones at the luteal phase. It was confirmed by a subsequent necropsy for the one case bearing with the luteal tissue in ovaries. The above data concurred with the othe reports indicated that giraffes adapt well in Taipei Zoo and the Fecal progesterone monitoring is a useful technique for examining the reproductive status of the giraffe. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。