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題 名 | 利用流動細胞分析儀偵測植生蟲草抗腫瘤多醣體(PN-2)對小白鼠巨噬細胞吞噬能力及輔助T淋巴細胞活性之影響 |
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作 者 | 李玲玟; 王正怡; 蘇慶華; | 書刊名 | 北醫學報 |
卷 期 | 23:1 1994.06[民83.06] |
頁 次 | 頁11-19 |
分類號 | 414.82 |
關鍵詞 | 植生蟲草水溶性多醣體; 免疫細胞活性; 流動細胞分析儀; PN-2; |
語 文 | 中文(Chinese) |
中文摘要 | 雙節棍胞子值生蟲草Phytocordyceps ninchukispora Su et Wang)是1985年於臺灣發現的新屬植生蟲草(Phytocordyceps)之單一種,由液態振盪培養液離心,取其上清液,經超微過濾器,分子篩及離子交換法可純化出水溶性多醣體(PN-2)。由過去之研究中得如PN-2具有頗似香菇多醣體(Lentinan)之抗腫瘤及免疫調節作用,除了能使植入小白鼠肺臟腫瘤的轉移受到阻礙,也能降低小白鼠皮下腫瘤惡化程度外,同時也具有免疫調節作用,可使小白鼠體內 T 淋巴細胞總數、輔助 T 淋巴細胞(Helper-T, Th)及巨噬細胞(macrophage)之數目增加。本實驗乃欲進一步探討PN-2除了增加上述細胞之數目外,是否也能促進其功能,故以T-依賴性抗原,即綿羊紅血球,注射到已投與PN-2的老鼠腹腔內,數日後採老鼠眼窩血,以微量紅血球凝集法(Microhemagglutination)測定其抗綿羊紅血球抗體之力價,結果發現實驗組與對照組小白鼠之抗體力價沒有差異,顯示PN-2對抗體之產生沒有影響。利用流動細胞分析儀(flow cytometer)分析巨噬細胞對已被螢光標示的白色念珠菌(Candida albicans)之吞噬能力,來測定 PN-2 對巨噬細胞吞噬能力之影響,結果發現PN-2可使巨噬細胞吞噬能力顯著增加,投與 PN-2 後其常在巨噬細胞(esident macro-phage)及硫代乙醇鈉激發之巨噬細胞(Thioglycollateelicited macrophage)與白色念珠菌結合之能力分別為41.11 ± 1.65%與61.55 ± 2.70%,對照組為29.88 ± 1.99%與41.28 ± 1.86%,扣除吸附於經疊氮化鈉(Sodium azide,NaN)處理過的巨噬細胞外之白色念珠菌, 得知投與 PN-2 後小白鼠常在巨噬細胞及硫代乙醇鈉激發之巨噬細胞吞噬白色念珠菌之能力分別為10.72 ± 0.53%與23.31 ± 0.39%,對照組為4.62 ± 0.92%與7.54 ± 0.92%,顯示PN-2能增加小白鼠常在巨噬細胞及硫代乙醇鈉激發之巨噬細胞之吞噬能力。由以上之結果,吾人推測PN-2可選擇性的作用於Th1,而非作用於Th2,促進其產生Interleukin-2 (IL-2)、Interferon-γ(IFN γ),進而活化巨噬細胞。 |
英文摘要 | Phytocordyceps ninchukispora Su et Wang is a new species of the Genus Phytocordyceps, dis-covered in Taiwan in 1985. Through the application of biochemical techniques, involving ultracentrifugation' gel filtration and ion-exchange chromatography, PN-2 was purified from the culture supernatant of Phytocordyceps ninchukispora Su et Wang. In the previous studies it has been found that PN-2 exhibits anti-tumor and immunomodulating activities. PN-2 inhibits lung metastasis of transplanted S-180 tumor and prevents further neoplastic change of cutaneous im-planted S-180 tumor in mice. PN-2 increase the number of total T lymphocyte' Thlymphocyte and monocyte/macrophage. All the above mentioned properties are shared by lentinan. In the present study we attempt to determine the possible influences of PN-2 on the function of Th lymphocyte and macrophage. Sheep red blood cell, an T-dependent antigen, was injected intraper-itoneal into mice which has been administrated with PN-2, the blood was removed sequentially from orbital plexus, and the serum hemagglutinin titer was measured by microhemagglutination. There was no difference in hemagglutinin titer in both experimental and control groups. These results indicate that PN-2 has no inf luence on antibody production against sheep red blood cell. The phagocytic activity of macrophage was determined by an in vitro method using flow cytometer to analyze the capacity of peritoneal macrophage to ingest florochrome (R-phycoerythrin)-labled Candida albicans. There is an obvious increase in the phagocytic activity of macrophage from PN-2 treated mice. The percentage of PN-2 treated resident and thioglycollate elicited macrophage bound/ingested Candida albicans were 41.11 ± 1.65 and 61.55 ± 2.70. and the control group were 29.88 ± 1.99 and 41.28 ± 1.86, respectively. The results were further corrected by deducting the amount of Candida albicans adhered on sodium azid treated cells from the original value. The net results of PN-2 treated resident and thioglycollate elicited macrophage to ingest Candida albicans were 10.72 ± 0.53% and 23.31 ± 0.39%, and the control group were 4.62 ±.0.92% and 7.54 ± 0.92%, respectively. The result indicates that PN-2 enhances the phagocytosis of mouse resident and thioglycoll ate elicited macrophage. Based on these results we propose that PN-2 seems to act selectively, it acts on Th1, but not on Th2, seceting lL-2 and IFN γ, which in turn activate macrophage. |
本系統中英文摘要資訊取自各篇刊載內容。