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題名 | Chloramphenicol投予鱸魚後以高效液相層析法檢測確認其殘留 |
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作者 | 呂車鳳; 劉猷士; | 書刊名 | 臺灣畜牧獸醫學會會報 |
卷期 | 61 1993.08[民82.08] |
頁次 | 頁73-82 |
分類號 | 439.5 |
關鍵詞 | 高效液相層析法; 殘留; 檢測; 鱸魚; |
語文 | 中文(Chinese) |
中文摘要 | 本研究以chloramphenicol(氯黴素;CP)分別藥浴及口服於鱸魚,然後以高效液 相層析法檢測魚肉中之殘留量,並以光二極體陣列式檢測器確認CP之殘留。依設定之抽取法 及分析條件,其結果如下:CP標準品分析靈敏度為0.2ppm,鱸魚肉中CP之分析靈敏度為 0.025ppm。鱸魚以CP 800pppm藥浴1天,停藥後魚肉中殘留之CP之0小時最高為1.22ppm, 此後即下降,至第7天可測得0.11ppm,第14天起測不出殘留(即低於0.025ppm);若以50mg/Kg body weight/day口服連續3天,則停藥後殘留之CP以12小時最高為29.45ppm,此後呈下 降趨勢,至第10天降至0.038ppm,第10天至28天內皆以低於0.05ppm之殘留量盤旋,於 第29天起測不出殘留(低於0.025ppm)。 |
英文摘要 | The residual level and withdrawal time of the currently used antibacterial agent chloramphenicol which was administered either by oral or by bathing in bass were determined with high performance liquid chromatography and confirmed with photodiode array detector. The respective analysis sensitivities of chloramphenicol in standard solution and bass meat were shown by the results to be 0.2 and 0.025 ppm. Chloramphenicol was given by bathing to bass at 80 ppm for one day. A peak residual level of 1.22 pm appeared on 0 hour after finishing the medication. After then, there were declining decreasingly in their residual levels and an amount of 0.11 ppm was detected on 7th day. No chloramphenicol (above 0.025 ppm) was demonstrated in the fish meat on 14th day after withdrawing the drug. On the other hand, chloramphenicol was given orally to bass at 50 mg per kg body weight daily for 3 consecutive days. The peak level of 29.45 ppm was shown on 12th hour and then the level was detected decreasingly until the lower level of 0.038 ppm on 10th day. There was a little bit residue under 0.05 ppm from 10th day. There was a little bit residue under 0.05 ppm from 10th day to 28th day. No chloramphenicol (above 0.025 ppm) was detected in the fish meat on 29th day after withdrawing the drug from the fish. |
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