頁籤選單縮合
題 名 | 栽培種茶樹原生質體分離 |
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作 者 | 廖麗貞; | 書刊名 | 高雄師大學報 |
卷 期 | 5 1994.03[民83.03] |
頁 次 | 頁268-244 |
分類號 | 434.817 |
關鍵詞 | 栽培種茶樹; 原生質體分離; |
語 文 | 中文(Chinese) |
中文摘要 | 茶樹(Camellia sinensis L.,O. Kuntz)屬山茶科(Theaceae)植物,屬嗜好類非 酒精性飲料作物,經濟價值極高且具有發展潛力之作物。茶樹的傳統改良方法需 花費極長之時間(至少10至12年),且變異有限,改良效果往往不佳。目前發展中 之原生質體融合、基因轉殖及遺傳工程等生物技術,不受轉殖植物常有之自交不 稔的限制,同時因茶樹可利用無性繁殖,故經由生物技術之方式來改良茶樹,頗 為理想。然而有關茶樹原生質體的分離、培養及融合等方面之研究,因茶樹原生 質體分離十分不易,迄今仍無正式的報告。本研究利用栽培種茶樹;青心烏龍、 台茶12號及台茶13號作為材料,進行茶樹原生質體分離條件的探討,期能分離得 到大量大小均一之原坐質體,以提供茶樹遺傳工程之材料。所獲結果摘要如后; 1.將葉片材料切成長條置於含有0.6M甘露醣醇,2(w/v)cellu1ase RS,2macerozyme R-10,0.5 pecto1yase Y-23及1PVP,pH5.8之酵素液中,置於25℃,5Orpm振盪情形下, 處理14小時,可得最高原生質體收量,其中以試管苗葉片為材料者,每克鮮重約 可獲得2.4x10�蔬茩鴠芺餕憿A而以盆栽土耕苗葉片為材料者每克鮮重只能分離得 到約3.0x10�雁茩鴠芺餕憿C 2.盆栽之土耕苗葉片材料在酵素處理之前,若先以1PVP溶液浸泡,可有效地提 高原生質體之分離量達 5.3x10�酥rotoplasts/g.Fw。而試管苗之葉片材料則無此必 要。 3.以試管苗各器官做為原生質體之分離材料,結果顯示,葉片可分離得到大量 之原生質體,子葉亦可以離得到少量之巨大原生質體,而根、莖或胚軸,無法分 離得到原生質體。 |
英文摘要 | Tea plant (Camellia sinensis L. O. Kuntz) is one of therepresentative nonalcoholic drinkable crops belongs to Tbeaceae. Teais very important and with high potential for commercial uses inTaiwan. Conventional breeding methods of tea plants are inefficientbecause of the prolonged time (minimum 10-12 years) and the limitedvariations.Obtaining plant regeneration from protoplasts enabling thetransfer of desirable gene from one clone to another by somatichybridization will bring an important subsidiary tool in breeding oftea plants in the future. But there is no new biotechnologiesassociated with protoplast isolation, regeneration and geneticengineering reported. This experiment is proposed to study the suitable methods forprotoplast isolation of tea cultivars: Chin-shin Oolong, TTE No.l2 andTTE No.lS. The results are summarized as follows :1. The leaves from potted and aseptical plantlets were used as materials for protoplast isolation. By using enzymatical methods, it is much easier to get larger amount of purified mesophvil protoplasts from aseptically cultured plants (2.4x10 protoplasts/g.F.W.leaf) than from potted plants (3x10�� protoplasts/g-F.W-leaf). The enzyme solution used was 0.6M mannitol, 2 (w/v) cellulase RS, 2 (w/v) macerozyme R-IO, 0.5 (w/v) pectolyase Y-23, and 1 (w/v) PVP, pH 5.8,and incubated at 25℃with ca. 50rpm shaking for 14 hours.2. The potted tea leaves presoaked in the 1PVP solution prior to enzymatical digestion promoted markedly protoplast isolation.3. Using the different tissues of aseptical tea plantlets as materials for protoplast isolation were tested. Among them, the mesophyll can be isolated the largest amount of protoplasts than cotyledons. Whereas, no protoplast was obtained from stem, hypocotyl and root. |
本系統中英文摘要資訊取自各篇刊載內容。