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題 名 | 用顆粒細胞和小白鼠受精卵或兩細胞早期胚胎行共同培養可增進其發育 |
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作 者 | 何信頤; 曾啟瑞; 張淑如; 簡立維; 陳庵君; | 書刊名 | 北醫學報 |
卷 期 | 22:2 1993.12[民82.12] |
頁 次 | 頁125-132 |
分類號 | 417.131 |
關鍵詞 | 顆粒細胞; 共同培養; 小白鼠胚胎; 體外受精; Granulosa cell; Coculture; Mouse embryo; IVF; |
語 文 | 中文(Chinese) |
中文摘要 | 我們取下小白鼠輸卵管內的兩細胞期胚胎和小白鼠顆粒細胞進行共同培養,發現胚胎形成囊胚的比例(75)明顯較沒有進行共同培養的(58)高,顯示顆粒細胞的共同培養對早期胚胎的生長發育有益。 在小白鼠體外受精的實驗方面,我們發現在3BSA溶液中的受精率(52)比在15FCS溶液中的受精率(22)為高,這可能是血清中含有較多不利精卵體外受精的物質。更進一步發現,使用microdrop in oil處理BSA溶液,受精率可顯著提高(70)。但體外受精所形成的早期胚胎,則不像取自受精於輸卵管的早期胚胎般可在體外發展至桑葚胚及囊胚。 由本實驗我們可以歸納出幾點結論:顆粒細胞的共同培養系統確可在體外協助早期胚胎的發展。另外,在進行體外受精時,使用無血清培養液比使用含血清培養液有較高的受精率。而microdrop in oil可提供比一般培養皿好的體外受精環境,使受精率更為提高。但這些體外受精所形成的受精卵,其胚胎品質比在輸卵管內受精的早期胚胎為差。 |
英文摘要 | In the experiment of coculture system with early embryo, a total of 478 2-cell stage embryos from oviduct of female mice were collected and cultured in BSA, FCS media or cocultured with murine granulosa cells. The rate of 2-cell mouse embryos reaching blastocysts was 65, 58, and 75 respectively and it was significant higher in the coculture group (p < 0.005). In the experiment of mouse in vitro fertilization (IVF), the fertilization rate in BSA (53) is much higher than in FCS (22), but the best fertilization rate (70) was observed when BSA medium was treated as microdrop in oil, compared with the control (53). However, further embryos growth derived from IVF could not develop as well as those fertilized in oviducts, and most of the fertilized eggs were arrested at the 2-cell stage. The supplementation of granulosa cells in coculture system also did not improve the embryos' growth and development. In conclusion: 1) coculture with granulosa cells may facilitate the development of mouse pre-implantation embryos; 2) microdrop in oil may provide a better environment for fertilization in vitro; 3) embryo quality is better if fertilized in oviducts rather than in vitro. |
本系統中英文摘要資訊取自各篇刊載內容。