查詢結果分析
來源資料
頁籤選單縮合
題 名 | Characterization of Thylakoid Membrane Inorganic Pyrophosphatase from Spinacia oleracia L=菠菜葉綠餅囊膜無機焦磷酸水解酵素之特性 |
---|---|
作 者 | 蘇哲弘; 曾驥孟; 江士昇; 潘榮隆; | 書刊名 | 嘉南學報 |
卷 期 | 19 1993.11[民82.11] |
頁 次 | 頁33-44 |
分類號 | 373.1 |
關鍵詞 | 焦磷酸水解酵素; 葉綠餅囊膜; 菠菜; Pyrophosphatase; Thylakoid; Spinach; |
語 文 | 英文(English) |
中文摘要 | 菠菜葉綠餅囊膜上存在有一無機焦磷酸水解酵素。此焦碳酸水解酵素可 在鹼性的環境中水解焦磷酸。而焦磷酸水解酵素之水解反應需要鎂離子,故而推 測其自然受質是鎂一焦磷酸。在研究陰離子對該酵素活性影響的測試中,發現僅 氟離子具強烈的抑制作用。此焦磷酸水解酵素的活性與光合電子傳遞及囊膜能激 狀無關。經輻射鈍化分析顯示該酵素在執行焦磷酸水解反應時之功能單位為86.9 ±8.4仟道爾吞。利用各種不同化學修飾劑分別對不同官能團進行修飾,發現該 酵素在活性中心處含有一半恍胺酸,且深埋於葉綠餅囊膜中。 |
英文摘要 | Biochemical properties of thylakoid membrane inorganic pyrophosphatase (PPase) from spinach were investigated in this study. Mg��+ (5 mM) markedly stimulated the PPase activity underalkaline pH values. The native substrate for the hydrolytic reaction of PPase appeared to bemagnesiuropyrophosphate. Among anions investigated, only did F cause severe inhibition. Illumination had no effect on PPase activity, indicating its independence of photosynthetic electrontransport and associated energization reactions. The radiation inactivation analysis showed thatthe functional size of PPase was 86.9 ± 4.3 kDa. Group specific modification of enzyme demonstrated the presence of essential sulfliydryl residue at the active site which was embedded in themembrane of thylakoid. |
本系統中英文摘要資訊取自各篇刊載內容。