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題名 | 家蠶原生質血球之形態與行為特性 |
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作者 | 張竣凱; 陳宏亮; 張光遠; | 書刊名 | 嘉南學報 |
卷期 | 19 1993.11[民82.11] |
頁次 | 頁73-84 |
分類號 | 438.38 |
關鍵詞 | 原生質血球; 黏附因子; 包圍作用; 防禦作用; Plasmatocyte; Encapsulation; Spreading; Adhesive factor; |
語文 | 中文(Chinese) |
中文摘要 | 以55 perctll配製之連續梯度分離家蠶血球。可得穩定性高及相當純度之 原生質血球(PL)。以掃描電子顯微鏡及位相差顯微鏡觀察,家蠶之原生質血球外 形為圓形或紡錘形,可迅速擴展開來而有多種形態變化。PL擴展行為之過程為(1) 血球延伸拉長與接觸面形成絲狀偽足,(2)在偽足間之細胞膜擴張形成板狀偽足, (3)PL極度扁平化。 家蠶顆粒血球內容物存有促進PL伸展偽足之黏附因子,此因子會促使PL擴展開 來黏附一起而參與包圍作用。此等黏附因子的作用在細胞性防禦反應中具有重要 之功能。 |
英文摘要 | Pure plasmatocytes (PL) were isolated by continuous density gradients of 55 hyper-osmotic percoll. Observation with phase contrast microscope and SEM showed these PLs were ovoidor spindle-shaped. In response to cell-substratum contact, these PLs underwent a serious of distinct morphological changes including 1). elongation of PLs 2). the production of filopodia andlamella. 3). fully spreading PLs with a flattened morphology. In vitro spreading assay showed the presence of cellular adhesive factors promoting the extension in PLs. This adhesive factor could mediate PL spreading and cause PL attaching to eachother to achieve encapsulation, thus it is suggested that this adhesive factor play an importantrole in cellular defense reaction. |
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