頁籤選單縮合
題 名 | 豬胚冷凍及其移置之研究 |
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作 者 | 郭有海; | 書刊名 | 中華農學會報 |
卷 期 | 163 1993.09[民82.09] |
頁 次 | 頁89-92 |
分類號 | 437.21 |
關鍵詞 | 冷凍; 移置; 豬胚; 冷凍豬胚; 冷凍保護劑; Frozen pig embryo; Cryoprotecfive agent; |
語 文 | 中文(Chinese) |
英文摘要 | Pig embryos seem to be extremely sensitive to cooling or freezing as with other animals. Although a technique for freezing pig embryos has been developed quite recently, our project compared the survival rate of the concentration of a cryoprotective agent (glycerol); different freezing media, and different stages of pig embryos. We then tried to transfer surviving frozen pig embryos to recipients. Donors and recipients were obtained from gilts 7-8 months of age, and mated or artificially inseminated during second estrus cycle. Embryos were collected 6 days after mating or artificial insemination by surgical operation. Blastocyst to hatched blastocyst pig embryos were frozen to -196°C; after freezing and thawing pig embryos were incubated in 37°C, 5% CO₂ or transferred to recipients. The results were: 1. PBS-containing 50% fetal calf serum (FCS), 15% egg yolk, 0.4% bovine serum albumin (BSA), and 1.5M glycerol had better survival rate than PBS containing 20% FCS and 1.5M glycerol used as the freezing solution for pig embryos. 2. Concentration of 1.5M glycerol had better survival rate than concentration 1.0M glycerol used as the cryoprotective agent for freezing pig embryos. 3. As to freezing and thawing pig embryos followed by in vitro culture, the survival rates of blastocyst, expanded blastocyst, hatching blastocyst and hatched blastocyst were 0.53, 38.5, and 73.2%, respectively. 4. As to survival of frozen pig embryos followed by in vitro culture transferred to 9 recipients, on day 117, one of the recipients was born one piglet (death) and 5 piglets (including 2 dead and 3 live) transferred from frozen (Yorkshire×Duroc) and fresh (Duroc×Duroc) pig embryos, respectively. The experiment indicated frozen pig embryos at the stage of expand blastocyst to hatched blastocyst had higher survival rate after in vitro culture, but there were extremely low farrowing rates when transferred to recipients. |
本系統中英文摘要資訊取自各篇刊載內容。