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頁籤選單縮合
題名 | The Application of Immunogold Labelling in the Diagnosis of Swine Pseudorabies Infection=免疫金粒子標示法在豬假性狂犬病診斷上之應用 |
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作者 | 陳三多; | 書刊名 | 臺灣畜牧獸醫學會會報 |
卷期 | 60 1992.12[民81.12] |
頁次 | 頁57-64 |
分類號 | 437.657 |
關鍵詞 | 免疫金粒子標示法; 狂犬病; 假性; 診斷; 豬; |
語文 | 英文(English) |
中文摘要 | 本實驗之目的,乃欲以免疫金粒子標示法標示出冷凍切片內之病毒,以做為診斷 毒性疾病之模式,本實驗中,以人工接種假性狂犬病病毒於四週齡之小豬,待其發病後,將 之犧牲,以病理解剖及切片檢查,證明其罹患豬假性狂犬病,然後,以肝臟組織做成冷凍切 片,經特異性抗體處理後,以適當濃度之蛋白質A金粒子加以標示,結果可在病毒造成壞死 的細胞內看到紅色金粒子沈澱物。 |
英文摘要 | The aim of this study is to indicate the pseudorabies virus particles in frozen section by immunogold lablelling, to act as a model for diagnosing viral disease. In this experiments, pseudorbies viruses were inoculated into 4 weeks old pigs. Then the experimental animals were sacrificed while the clinical signs appeared or the pigs died, necropsies were performed carefully, histopathologic section was also examined in order to prove that these animals were died from virus attack. Liver frozen sections were treated with specific antiserum of pseudorabies, and labeled with protein A gold. A reddish coloration was seen in virus infected tissue. |
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