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題 名 | Characterization of Superoxide Dismutase in Arabidopsis=阿拉伯芥超氧歧化酶的研究 |
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作 者 | 潘素美; 姚元宇; | 書刊名 | 國立臺灣大學理學院植物學系研究報告 |
卷 期 | 37:1 1992.03[民81.03] |
頁 次 | 頁58-66 |
分類號 | 371.8 |
關鍵詞 | 阿拉伯芥; 超氧歧化酶; Arabidopsis; Superoxide dismutase; |
語 文 | 英文(English) |
中文摘要 | 以膠體電泳法分析阿拉伯芥不同生長階段的葉片,莖,花及以paraquat處理過植株之超氧歧化酶(SOD)。並以CN-和H2O2對超氧歧化酶不同程度之抑制作用來鑑定其同功酶。阿拉伯芥不同年齡的葉片及不同器官之SOD同功酶形式相似,都具有Mn-SOD,Fe-SOD和CuZu-SOD,其分子量分別為80KD, 50 KD, 35KD 25KD及20KD;等電點為pH3.8, 4.5, 4.8及5.35。CuZn-SOD為阿拉伯芥超氧歧化酶中活性最大之一群,不同生長階段的葉片,莖,花之活性雖沒有明顯變化;然而paraquat處理過之植株CuZn-SOD III活性卻明顯增加。 |
英文摘要 | Superoxide dismutase (SOD: EC 1.15.1.1) from different plant parts of Arabidopsis and paraquat-treated leaves were characterized using polyacrylamide gel electrophoresis. Five different forms of SOD were identified by their sensitivity to cyanide and hydrogen perioxde. The molecular weight of Mn-SOD, Fe-SOD and CuZn-SODs was estimated to be 80KD, 50 KD , 35KD 25KD, 20KD and 15KD, respectively. A similar pattern of Mn-, Fe- and CuZn-SOD was observed in all plant parts. The pIs of the SODs were determined to be 3.8, 4.5, 4.8 and 3.35 by isoelectric focusing. CuZn-SODs contributed the major activity of total SOD. Only the CuZn-SODIII activity showed an apparent increase following the Arobidopsis plants treated with paraquat. |
本系統中英文摘要資訊取自各篇刊載內容。