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題名 | 快速乳液凝集法與傳統方法鑑定金黃色葡萄球菌之比較 |
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作者姓名(中文) | 陳繼祥; 周明淵; | 書刊名 | 中華民國小兒科醫學會雜誌 |
卷期 | 32:5 民80.09-10 |
頁次 | 頁297-302 |
分類號 | 414.83 |
關鍵詞 | 方法; 比較; 快速; 乳液; 金黃色; 傳統; 葡萄球菌; 凝集法; 鑑定; |
語文 | 中文(Chinese) |
中文摘要 | 快速乳液凝集法-staphaurex(Wellcome diagnostics)與傳統的凝固酶(coagulase)對熱安定核酸酶(thermo-stable nuclease)、核酸酶(nuclease)、木蜜醇(mannitol)醱酵反應等比較,來鑑定金黃色葡萄球菌。測試503株葡萄球菌,其中金黃色葡萄球菌(Staphylococcus aureus)277株,表皮葡萄球菌(Staphylococcus epidermidis)201株,腐生性葡萄球菌(Staphylococcus saprophyticus)25株。敏感性爲staphaurex99.6%,兔血漿99.6%,人血漿99.2%,對熱安定核酸酶100%,核酸酶100%,木蜜醇醱酵反應98.9%。專一性爲staphaurex99.6%,免血漿100%,人血漿100%,對熱安定核酸酶98.7%,核酸酶95.7%,木蜜醇醱酵反應91.1%。staphaurex會與某些腐生性葡萄球菌呈假陽性反應。 |
英文摘要 | Rapid latex agglutination assay, staphaurex (Wellcome Diagnostics) was compared with the tube coagulase, thermo-stable nuclease, deoxyribonuclease and mannitol fermention test for the identification of Staphylococcus aureus, A total of 277 clinical isolates of Staphylococcus aureus, 201 Staphylococcus epidermidis, and 25 Staphylococcus saprophyticus were tested. The results showed that sensitivities were: staphaurex, 99.6%; rabbit plasma, 99.6%; human plasma, 99.2%; thermostable nuclease, 100%; deoxyribonuclease, 100%; mannitol fermentaton, 98.9%; as for specificities results showed: staphaurex, 99.6%; rabbit plasma 100%; human plasma, 100%; thermo-stable nuclease, 98.7%; deoxyribonuclease 95.7%; mannitol fermentation, 91.1%; respectively. In our study, staphaurex is recommended because it is simple to operate, save in time and economic in cost. |
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