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題名 | 臺灣種豬感染豬棒狀桿菌(C. suis) 之研究 (2):形態學及生長需求特性 |
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作者姓名(中文) | 林一志; 吳福明; 盧文鴻; | 書刊名 | 臺灣畜牧獸醫學會會報 |
卷期 | 58 1991.12[民80.12] |
頁次 | 頁69-80 |
分類號 | 437.243 |
關鍵詞 | 生長; 形態學; 感染; 種豬; 臺灣; 需求; 豬棒狀桿菌; |
語文 | 中文(Chinese) |
中文摘要 | 在1989年10-12月間,自本省中、南部二種豬場之膀胱炎和腎孟腎炎母豬與健 康種公豬所分離到的19株豬棒狀桿茵,在實驗室中進行形態學及生長需求特性的研究。豬棒 狀桿菌為革蘭氏陽性菌,易褪色,多形態之桿菌,呈柵狀排列或粘著聚集狀。觀察血液培養 基上的單獨菌落,24小時後,可達到直徑0.5mm大小,二天後,菌落直徑為1.0-2m,菌落外 觀形態為典型之鋸齒狀邊緣,中央部份凸起,7天後菌落直徑可達3-5m,菌落變平。添加低 濃度的尿素或碳水化合物至液體培養基中可以提升其生長性能。最適宜的溫度為37°C; 最 適宜的氣體環境則為在厭氧下(Oxoid anaerobic system)生長最好,但實驗結果顯示:此菌 在好氧環境下仍然可以生存一段長久時間,但生長並不好,可見C.suis並不是一株絕對厭氧 菌。 |
英文摘要 | Nineteen Corynebacterium suis isolates, one isolate from a sow with cystitis and pyelonephritis from a pig farm at the southern Taiwan in Oct. 1989 and eighteen isolates from clinically healthy boar's preputial diverticuli, were studied on bacterial morphology and growth requirement in our laboratory. C. suis was a Gram's positive, poorly stainable polymorphic bacillus The slide smear from the cultured colony showed palisading or aggregated arrangement. On the blood agar, the diameter of a isolated colony was about 0.5mm at 24 hours and reached to 1.0 to 2.0mm at 48 hours. The colony has a typical sawtoothed edge with raised center. The coliny became flattened and grew up to 3 to 5mm in diameter on 7th day. The bacterial growth rate would improve by adding slinght amount of urea or carbohydrate into liquid media. The most optimum culture condition for C. suis was 37℃ in oxoid anaerobic system but under the aerobic environment the bacteria was still able to survive for a long period of time. The C. suis was not an absoloute anaerobic orgamism. |
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