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題名 | 利用酵素連結抗體免疫法(ELISA) 檢定水稻穀粒離層酸含量 |
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作者姓名(中文) | 陳宗禮; 周煒裕; 林金和; | 書刊名 | 中華農學會報 |
卷期 | 155 1991.09[民80.09] |
頁次 | 頁57-63 |
分類號 | 434.113 |
關鍵詞 | 水稻; 免疫法; 抗體; 連結; 酵素; 穀粒; 離層酸; |
語文 | 中文(Chinese) |
中文摘要 | 應用免疫反應測定植物生長調節劑是目前研究的趨勢之一,尤其以ELISA檢定,不但簡便準確,又安全快速,同時可以進行大量篩檢工作。利用Idetek公司所發展的ABA單株抗體,可以很精確地測度植物組織中游離態ABA含量。測定組織中ABA含量必須以適當稀釋溶液行才可以得到合理準確的結果。 利用ABA-ELISA測定乳熟期與黃熟期水稻穀粒中游離ABA含量,其結果如下:在二時期中,穎果所含ABA量皆較穀殼中高,在乳熟期,每一穎果含1.61pg ABA,殼僅有0.068pg;在黃熟期,每一穎果含0.093pg ABA,而殼中含0.034pg。游離態ABA含量隨殼粒成熟而減少,此種現象在穎果中最明顯,乳熟期穎果中游離態ABA濃度為100.42 ng/g.f.w,至熟期減少為5.31 ng/g.f.w,穀殼中的變化較小。隨著殼粒的發育,可能大部分游離態ABA轉變為結合態,這種游離態ABA隨發育期變化的現象可能 和種子成熟的生理代謝有密切關係。 |
英文摘要 | The monoclonal antibody from ldetek which was raised against (±)ABA(C-4’) BSA are commercially available. It is reported to recognize only free (±) ABA. Used ELISA based on competitive binding free and enzyme-linked ABA, they give estimates of free ABA in crude extracts of rice grains. The best estimates of free ABA were obtained from adequate dilute of extract solutions. Free ABA was determined by ELISA during milky stage and yellow-ripe stage in rice grains. The results showed that free ABA in grains distributed much in caryopsis than the hulls. They were 1.61 pg and 0.068 pg per grain, respectively. Free ABA per grain decreased from 1.678 pg to 0.127 pg during the investigated period. The concentration of free ABA at milky stage was higher, 100.42 ng/g. f.w, and then dropped significantly to 5.31 ng/g.f.w, at yellow-ripe stage. Dramatic change of free ABA levels in developing rice kernel might play a significant role to regulate seed metabolic status. |
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