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題 名 | Cardiovascular Effects of Neuropeptide Y in Rat Brainstem Nuclei=神經胜肰Y 在老鼠腦幹核內之心臟血管作用 |
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作 者 | 曾清俊; 童吉士; | 書刊名 | 醫學研究 |
卷 期 | 11: 附輯1 民80.04 |
頁 次 | 頁55-66 |
分類號 | 364.6791 |
關鍵詞 | 心臟; 老鼠; 血管; 神經胜冴; 腦幹核內; |
語 文 | 英文(English) |
中文摘要 | 中樞兒茶酚胺神經元己知與心臟血管管制有關,而神經胜肽Y(NPY)被發現與腎上腺素及新腎上腺素同時存在腦組織內。本篇文章討論了NPY在中樞心臟血管管制上所扮演之角色。雄性S-D大白鼠,以Urethane麻醉,行動靜脈插管以記錄血壓,心跳及給藥。動物固定於立體定位儀上,局部顱骨切開術後,微量注射(60nl)不同濃度之NPY,NPY抗體,α₂作用劑或對抗劑至腦幹特定區域,觀察其對血壓、心跳的影響。單側微量注射NPY至孤立束核(NTS)可產生劑量依賴性的降壓及降心跳作用。在最後區,可以產生快速的升壓及心跳加快反應,隨後有較長時間的降壓及降心跳作用。至於在腦幹頭側腹區,則產生升壓及降心跳的作用。實驗過程中,為了測試NPY之專一性作用,我們以其抗體微量注射至NTS,可完全的抑制NPY的作用,而去除活性之抗體則不具此作用。微量注射α₂對抗劑,idazoxan及yohimbine可抑制NPY在NTS所產生的降壓及降心跳作用;另一方面,NPY之抗體則可顯著的抑制中樞降血壓藥物,α-MNE及clonidine之降壓作用。本實驗結果顯示NPY在腦幹核內有複雜的心臟血管作用,且可能與兒茶酚胺在中樞心臟血管管制上有相互作用。 |
英文摘要 | Central catecholaminergic neurons are involved in cardiovascularcular regulation. Neuropeptide Y (NPY) coexists with adrenaline and noradrenaline in the rat brain. This review article discuss the possible role of NPY in entral cardiovascular control. Male Sprague-Dawley rats were anesthetized with urethane, and blood pressure was monitored intra-arterially. Intramedullary microinjection (60 nl) of NPY, antibody, α₂ agonists and antagonists were made into the nucleus tractus solitarii (NTS), Into the area postrema (AP), and into the rostral ventrolateral medulla (VLM). Injection site was identified by L-glutamate administration and confirmed histologically. Unilateral injection of NPY into the NTS produced a prominent dose-related decrease in heart rate and blood pressure. Injection into the AP produced an initial increase in heart rate and mean blood pressure followed by a prolonged decrease in heart rate and mean blood pressure. However, injection of NPY into the rostral VLM produced a doser-elated increase in blood pressure and decrease in heart rate. To test the specific effect of NPY, rabbit anti-NPY antiserum was used. Prior administration of the antibody inhibited the effects of subsequent administration of NPY, whereas administration of inactivated antibody was not able to prevent these effects. Administration of the α₂ antagonists, idazoxan or yohimbine, prior to the microinjection of NPY attenuated the potent depressor and bradycardic effect of NPY in a dose-dependent manner. Similarly, prior administration of the NPY antibody attenuated the depressor effect of the central antihypertensive agents, alpha-methylnorepinephrine (α-MNE) and clonidine, whereas administration of inactivated antibody or control normal rabbit serum were not able to attenuate these effects. More interestingly, a subthreshold dose of NPY could potentiate the effect of α-MINE. These results suggest that exogenously applied NPY has distinct cardiovascular effects in the brainstem nuclei, and NPY might interact with catecholamines in central cardiovascjlar regulation. |
本系統中英文摘要資訊取自各篇刊載內容。