查詢結果分析
來源資料
相關文獻
- Selection of cell lines with high DAPI stainability from rice (Oryza sativa L.) suspension culture for protoplast isolation and regeneration
- 利用水稻懸浮細胞分離原生質體之研究
- 稗子原生質體之分離與培養
- 巒大杉之癒合組織誘導及原生質體分離
- 臺灣櫸細胞懸浮培養與原生質體分離之研究
- 水稻與稗子原生質體融合與培養
- 水稻品種間原生質體融合培養及植株再生
- 利用營養細胞培養法促進水稻原生質體生長
- Cell Suspension and Protoplast Isolation of Eucalyptus Macarthurii and E. radiata
- Tissue culture investigations of bamboo (5):Recovery of callus from protoplasts of suspension-cultured Bambusa cells
頁籤選單縮合
題 名 | 利用水稻懸浮細胞分離原生質體之研究 |
---|---|
作 者 | 陳彥雄; 劉麗飛; 賴光隆; | 書刊名 | 中華農學會報 |
卷 期 | 149 1990.03[民79.03] |
頁 次 | 頁1-16 |
分類號 | 434.28 |
關鍵詞 | 分離; 水稻; 原生; 細胞; 質體; 懸浮; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究乃利用臺南5號(TN5)水稻懸浮培養細胞為材料,進行原生質體分離探討。試驗結果摘要如下: 1.以繼代培養後5~7天的懸浮培養細胞,置於4% cellulase R-10和0.5% macerozyme R-10的混合溶液中,添加0.5M mannitol為滲透壓劑,於28℃下靜置5小時後,可以分離出大量的原生質體,分離量可達1×10⁶原生質體/毫升酵素液。 2.連續每隔2天更換新鮮的懸浮培養液,共換3次,可明顯提高懸浮培養細胞的生長,降低細胞團粒大小,進而促進原生質體之分離。 3.在酵素溶液中添加適量的serine、slycine或methionine等胺基酸,有利於原生質體之分離。 4.懸浮細胞的培養狀況,酵素、滲透壓劑、以及胺基酸等均會影響原生質體之分離,如能針對這些因子尋求最適當的分離條件,將可產生豐富的原生質體,以供給進一步培養試驗之用。 |
英文摘要 | This experiment was conducted to study the isolation of rice protoplasts. The results obtained are summarized as follows: 1. Suspension cultured rice cells were used as materials for protoplast isolation. Cells cultured for 5 to 7 days after subculture were suitable for protoplast isolation. Protoplasts were isolated by enzymatical method, that is cells were mixed with enzyme solution containing 4% cellulase R-10, 0.5% macerozyme R-10 and 0.5 M mannitol, and then incubated at 28°C for 5 hrs. The yield of protoplasts were about 1×10⁶ protoplasts/ml cells. 2. Changing the suspension medium with fresh medium every other day for three times will promote the growth of suspension cells, increase the percentage of small cell clumps and the protoplast yield. 3. Proper amounts of serine, glycine or methionine added to the enzyme solution are helpful to the isolation of protoplasts. 4. It is suggested that the conditions of suspension cells, enzyme and osmoticum concentration, and the kind of amino acid can influence the isolation of protoplasts, it is necessary to find the proper methods of isolation that could produce more protplasts fro the future experiments. |
本系統中英文摘要資訊取自各篇刊載內容。