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題名 | Transmission of Rice Wilted Stunt Virus By Brown Planthopper (Nilaparvata lugens Stal)=褐飛蝨傳播水稻萎凋矮化病病毒之研究 |
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作者姓名(中文) | 陳慶忠; 邱人璋; | 書刊名 | 臺中區農業改良場研究彙報 |
卷期 | 23 1989.06[民78.06] |
頁次 | 頁3-10 |
分類號 | 434.111 |
關鍵詞 | 水稻; 病毒; 萎凋矮化病; 傳播; 褐飛蝨; |
語文 | 英文(English) |
中文摘要 | 水稻凋矮化病於民國66年10月在台中縣東勢鎮首次發現。本病由褐 飛蝨(Nilaparvata lugens Stal)以持續性方式傳播。在28∼30℃下,病毒在蟲體內 之潛伏期平均為6.7日(3∼14日)。供試蟲群獲毒率為41%(22.2∼64.4%)。最短 獲毒及接吸食時間分別為60及30分鐘。在15∼35℃溫度範圍內,媒介昆蟲均 能獲毒,獲毒後之潛伏期隨溫度增高而縮短,但當溫度高於30℃以上時,媒介 昆蟲死亡亦隨之增加。本病毒不能經卵或以機械方式傳播。在25±1℃定溫下, 利用生物檢定法及酵素聯結法同時檢定吸食病株後之褐飛蝨,發現酵素聯結法最 早於吸毒後第五日偵測到病毒;而生物檢定法則於第7日檢定到帶毒病蟲。顯然 酵素聯結法比生物檢定法能較早期於帶毒蟲體內偵測到病毒。 |
英文摘要 | Rice wilted stunt (RWS) was first found in central Taiwan in 1977. The disease, transmissible by the brown planthopper Nilaparvata lugens (Stal) in the persistent manner. N. Lugens transmitted RWSV with a incubation period of 6.7 days (range 3-14 days) at 28-30℃ Allowed an acquisition access of 24 hr, about 41% (22 to 64%) of the test insects became transmitters. The minimal acquisition and inoculation access periods were detected as 1 hr and 30 min, respectively. The insects were able to acquire RWSV at temperature range of 15-35℃ and after acquisition feeding, the incubation period was shorten as increase in temperature, through temperature up to 30℃, mortality of the test insect was higher. Neither transovarial passage nor mechanical transmission were observed. Under 25±1℃ constant temperature, bioassay and ELISA were used to detect the initial transmission time and the changes of concentration of the RWSV. Results indicated ELISA started to detect RWSV in the insect vector at 5 days after acquisition and bioassay first identified the virus transmitter at 7 days after acquisition feeding, therefore ELISA might be a more sensitive method to detect RWSV in the insect vector. |
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