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題名 | 水稻原生質體培養及植株再生 |
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作者 | 劉麗飛; 王國忠; 賴光隆; | 書刊名 | 中華農學會報 |
卷期 | 147 1989.09[民78.09] |
頁次 | 頁1-9 |
分類號 | 434.113 |
關鍵詞 | 水稻; 再生; 原生; 培養; 植株; 質體; |
語文 | 中文(Chinese) |
中文摘要 | 本試驗利用水稻品種臺南5號之幼胚癒合組織,做成懸浮培養細胞,以AA培養基培養,固定每週繼代培養一次。於繼代培養後3至7天,收集細胞進行原生質體之分離與培養。結果顯示,當懸浮細胞在AA培養基內3至9個月,最有利於原生質體培養,雖然大部分原生質體在一週內死亡,部分原生質體進行不正常生長,並在二週內崩解消失,但是少部分原生質體則可進行正常細胞分裂,並於四週內形成小細胞團。其平埋效率明顯受熱休克處理,培養基滅菌方式,及繼代培養之影響,目前最高可達0.7%。將小細胞團移至分化培養基,則有芽體之分化,並形成幼植珠,其分化率約在1.5-4.5%。隨懸浮培養時間之增加,幼植株分化率有降低之趨勢。本試驗顯示水稻原生質體可經培養,並再生為植株,進一步將探討如何提高培養效率,並利用細胞轉形之方法,嘗試水稻改良之研究。 |
英文摘要 | Rice (Oryza sativa L. cultivar Tainan 5) cell suspension was established from callus induced from young embryos. The suspension cells were cultured in AA medium, an subcultured at an interval of 7 days. After subculturing 3 to 7 days, cells were collected for protoplast isolation and culture. The results showed that protoplasts can be cultured successfully only when they were isolated from suspension cells maintained in AA medium for 3 to 9 months. During protoplast culture, most protoplasts died within one week, some protoplasts proceeded abnormal cell division and lysed within 2 weeks. Only small part of protoplasts proceeded normal cell division and formed cell colonies after 4 weeks. The culture efficiency was apparently affected by heatshock treatment, the sterilization methods of protoplast culture medium and the condition of protoplast subculture. At present,the highest culture efficiency was 0.7%. Plants can be regenerated from cell colonies after being transferred to regeneration medium. The frequency was about 1.5-4.5%. It showed decrease tendency when suspension cell were cultured for a longer period. Further studies will be conducted to increase the culture efficiency and to try the cell transformation for rice variety improvement. |
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