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| 題 名 | 人體表皮細胞培養 |
|---|---|
| 作 者 | 方榮煌; | 書刊名 | 中華民國外科醫學會雜誌 |
| 卷 期 | 20:5 1987.09[民76.09] |
| 頁 次 | 頁425-430 |
| 分類號 | 416.413 |
| 關鍵詞 | 人體; 表皮細胞; 培養; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 自民國75年初開始,我們卽著手於建立人體表皮細胞培養之技術。目前已可成功地自病人身上取得一小塊約1-2 cm2之表皮後,經11-13天培養或多塊75 cm2之表皮層(epidermal sheet),並可繼續大量次培養(subculture)。培養成功之表皮層,經蘇木精及伊紅染色法(hematoxylin and eosin, HE, stain)及螢光抗體染色法,顯示出上皮細胞(epithelial cell)之形態與特徵;又經電子顯微鏡切片觀察為多層之構造。本文僅探討影響培養表皮細胞生長之因素及報告我們的經驗。至於臨床應用正俟機進行中。 |
| 英文摘要 | Since early 1986, we established the technique of human epidermal cell culture. Work in our laboratory has now resulted in reproducible methods for the serial growth of large amounts of multilayered human epidermal sheets in 11-13 days from a small piece of epidermis about 1-2 cm2 in size. The cultured epidermal cells show epithelial cell morphology after staining with hematoxylin and eosin. The presence of keratin and the stratified construction of cultured epidermal cells was shown by the immunofluorescent method and the electron micrograph. Factors affecting the growth of epidermal cell culture and our experience in epidermal cell culture were discussed. |
本系統中英文摘要資訊取自各篇刊載內容。