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| 題 名 | 急性熱緊迫對L2品系臺灣土雞公雞下視丘基因表現與DNA甲基化之影響=Effect of Acute Heat Stress on Gene Expression and DNA Methylation in the Hypothalamus of Male L2 Strain Taiwan Country Chickens |
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| 作 者 | 薛凰均; 莊子萱; 鄭淳予; 李璦綸; 陳泓霖; 鄭皓騰; 陳志峰; 陳洵一; 黃三元; | 書刊名 | 農林學報 |
| 卷 期 | 70:4 2023.12[民112.12] |
| 頁 次 | 頁271-294 |
| 分類號 | 437.71 |
| 關鍵詞 | 臺灣土雞; 急性熱緊迫; 下視丘; 轉錄體; DNA甲基化; Taiwan country chickens; Acute heat stress; Hypothalamus; Transcriptome; DNA methylation; |
| 語 文 | 中文(Chinese) |
| DOI | 10.30089/JAF.202312_70(4).0004 |
| 中文摘要 | 熱緊迫使畜禽生長與生殖性能低落,下視丘為禽類體溫調節中樞,可以偵測外在環境溫度變化,環境熱緊迫也 可能改變動物體內表觀遺傳修飾而使動物耐過或適應不利的生存環境,本研究之目的為探討急性熱緊迫對 L2 品系台 灣土雞公雞下視丘基因表現之影響以及 DNA 甲基化修飾是否造成基因差異表現。本研究使用 197 隻 30 週齡 L2 品系 台灣土雞公雞,其中 192 隻雞接受 38℃及相對溼度 55%的急性熱緊迫處理四小時,另五隻雞作為對照組,飼養在 25℃ 及相對溼度 55%環控氣候室;熱緊迫處理後,選取加熱前後體溫差≧6.5℃者作為熱敏感組及體溫差≦2.5℃者作為熱抵 抗組,試驗結束後每組取五隻雞採下視丘供後續分析。L2 品系公雞下視丘急性熱緊迫後之 mRNA 表現、DNA 甲基化 程度定量與定性分析分別以 RNA 定序(RNA sequencing, RNA-seq) 與焦磷酸定序(pyrosequencing, Pyro-seq)進 行。急性熱緊迫處理後公雞下視丘差異表現基因(differentially expressed genes, DEGs)中有 295 個基因表現量顯著 增加與 131 個基因表現量顯著減少;基因功能(gene ontology)註解結果顯示此等差異表現基因主要參與細胞過程、 代謝過程及生物性調節等生物過程;急性熱緊迫顯著改變下視丘中熱緊迫蛋白家族( HSPB1 、 HSPB8 、 HSPB9 及DNAJA4)、抗氧化酶( GPX1)、下視丘緊迫調節( FKBP5)與 DNA 甲基化與轉錄因子結合蛋白質( DNMT3A 及 CREBBP) 之 RNA 表現量。Pyro-seq 結果顯示熱敏感組雞隻 HSPB8、 GPX1 與 DNMT3A 啟動子內特定 CpG 位點之甲基化程 度顯著高於熱抵抗組者。本研究結果顯示急性熱緊迫處理改變 L2 品系台灣土雞公雞下視丘與細胞過程、代謝過程及 生物性調節相關基因 mRNA 之表現,雞隻在應對急性熱緊迫時其 HSPB1、 HSPB8 和 GPX1 的 DNA 甲基化的變化存 在明顯差異,然此等甲基化改變對耐熱能力影響之確切機制仍有待進一步證實。 |
| 英文摘要 | Heat stress causes poor growth performance and reproductive performance in farm animals. The hypothalamus acts as a crucial center to regulate body temperature and detect environmental temperature changes. Heat stress may alter epigenetic modifications to allow animals withstand or adapt to the environment stress. The purpose of this study was to investigate the effect of acute heat stress on gene expression and to explore whether the modification of DNA methylation affects gene expression in layer-type L2 strain Taiwan country chickens (TCCs). A total of 197 30-week-old roosters of L2 strain TCCs were used in this study. One hundred and nighty-two roosters were acute heat-stressed at 38°C and 55% RH for 4 h. The other five roosters were kept at 25°C and 55% RH as a control group (CTL). After acute heat stress, the roosters with body temperature change before and after heat stress higher than 6.5°C were grouped as sensitive group (SEN) and those with body temperature change less than 2.5°C were grouped as resistant group (RES). The hypothalamus of five roosters in each group were collected for RNA sequencing (RNA-seq) and DNA methylation analysis by pyrosequencing (Pyro-seq). There were 295 genes upregulated and 131 genes downregulated among the differentially expressed genes (DEGs) after acute heat stress in the hypothalamus of L2 strain TCCs. Gene ontology annotation indicated that most of the DEGs were involved in biological processes of cellular process, metabolic process and biological regulation. The expressions of genes in HSP family ( HSPB1, HSPB8, HSPB9 and DNAJA4), antioxidant ( GPX1), hypothalamus function ( FKBP5), and DNA methylation and transcription ( DNMT3A and CREBBP) were significantly changed and were selected for further DNA methylation analysis. Result of Pyro-seq revealed that the methylation level of specific CpG site in HSPB8,GPX1 and DNMT3A were higher in SEN group than RES group. The results of this study showed that acute heat stress changed the mRNA expression of genes related to cellular process, metabolic process and biological regulation in hypothalamus of L2 strain TCCs. There were differences in DNA methylation between SEN and RES roosters in response to acute heat stress. However, the exact mechanism of their effect on heat resistance requires further verification. |
本系統中英文摘要資訊取自各篇刊載內容。