查詢結果分析
來源資料
頁籤選單縮合
| 題 名 | 產蛋雞長期採食低劑量嘔吐毒素對腸道組織與蛋中殘留量之影響=The Long-term Effects of Low-dose Deoxynivalenol on Intestinal Tissues and Transmission into Eggs for Laying Hens |
|---|---|
| 作 者 | 烏仕明; 魏恒巍; | 書刊名 | 中國畜牧學會會誌 |
| 卷 期 | 52:1 2023.03[民112.03] |
| 頁 次 | 頁1-21 |
| 分類號 | 437.71 |
| 關鍵詞 | 嘔吐毒素; 腸道; 雞蛋; 產蛋雞; Deoxynivalenol; Intestine; Chicken eggs; Laying hens; |
| 語 文 | 中文(Chinese) |
| DOI | 10.30194/JCSAS.202303_52(1).0001 |
| 中文摘要 | 本實驗旨在探討產蛋雞長期採食低劑量嘔吐毒素(deoxynivalenol, DON),對腸道組織與蛋中嘔吐毒素殘留量之影響,並檢測毒素是否會造成慢性傷害。試驗共分為 7 組,嘔吐毒素之添加量分別為 0、35、70、140、280、560 或 1,120 ppb,每組含 12 隻 12 週齡之海蘭品系 W-36 單冠白色來航雞。試驗期間,飼糧與飲水任飼,每日接受人工光照 16 hr,每天記錄產蛋與否、蛋重與採食量。結果顯示,長時間採食含低劑量嘔吐毒素飼糧之產蛋雞,對其體重、自 19 週齡開始之每 7 週平均隻日產蛋率與飼料換蛋率,均無顯著影響;但連續 28 週食用含有嘔吐毒素 1,120 ppb 之飼料時,雞隻採食量則顯著下降。對照組之蛋殼強度顯著高於 280、560 與 1,120 ppb 之處理組者(P < 0.05)。粗蛋白、灰分與乙醚萃取物之消化率,無論在濃度或時間之效應,皆不顯著,且以乾基為基礎之排泄物菌落數在各組間也差異不顯著。試驗結束時,檢測毒素對於小腸絨毛面積、絨毛長度、腺窩深度及絨毛長度對腺窩深度之比值,不同處理組間無顯著差異存在。雞隻血漿中之天冬胺酸轉胺酶、丙胺酸轉胺酶與鹼性磷酸酶之活性以及白蛋白之濃度,並未出現顯著差異;然而在第 28 週時,飼糧中含有1,120 ppb 嘔吐毒素之處理組,血中總蛋白質濃度顯著較對照組者為低,且對照組腎臟對體重之比例顯著高於 280、560 與 1,120 ppb 之處理組者;但在肝臟或脾臟對體重之比例,各組間差異不顯著。以高效液相層析儀進行檢測處理組之樣品發現,在蛋中與血液中,並無嘔吐毒素存在,且在排泄物中含量極低;對照組之排泄物中亦無嘔吐毒素存在。換算毒素之消化率,則可以發現餵飼嘔吐毒素濃度 1,120 ppb 之處理組於實驗第 1 週時,顯著大於 35 與 70 ppb 之處理組者(P < 0.05)。綜觀本試驗之結果,產蛋雞長期食用含低劑量嘔吐毒素之飼料,並不會影響其所生產雞蛋之食品安全性,也不影響到產蛋率及飼料換蛋率;但在 40 週齡時,蛋殼強度會隨飼糧中低劑量嘔吐毒素濃度之大幅提升而呈現顯著下降;對雞隻之健康方面有些許的影響,但程度不大。 |
| 英文摘要 | This experiment aimed to investigate the impact of long-term consumption of low-dose deoxynivalenol (DON) on the intestinal tissues and residual levels of DON in eggs, as well as to examine whether the toxin causes chronic damage. The experiment consisted of seven groups with DON concentrations of 0, 35, 70, 140, 280, 560, or 1,120 ppb, each group containing 12 White Leghorn W-36 single-comb layers at 12 weeks of age. Throughout the trial, the chickens had ad libitum access to feed and water, received 16 hours of artificial light daily, and daily records were kept for egg production, egg weight, and feed intake. The results showed that long-term consumption of feed containing low-dose DON did not significantly affect body weight, average daily egg production from 19 weeks of age onwards, and feed conversion ratio. However, a significant decrease in feed intake was observed in the group continuously fed with feed containing 1,120 ppb of DON for 28 weeks. The control group exhibited significantly higher eggshell strength than the 280, 560, and 1,120 ppb treatment groups (P < 0.05). Digestibility rates of crude protein, ash, and ether extract, regardless of concentration or time effects, showed no significant differences. The colony count of excreta microorganisms on a dry basis also exhibited no significant differences among the groups. At the end of the experiment, measurements of small intestinal villus area, villus height, crypt depth, and the villus height to crypt depth ratio revealed no significant differences among the treatment groups. Enzyme activities of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and albumin concentration in chicken plasma did not show significant differences. However, at week 28 of the experiment, the group fed with feed containing 1,120 ppb of DON exhibited a significantly lower total protein concentration in the blood compared to the control group. The kidney-to-body weight ratio in the control group was significantly higher than that in the 280, 560, and 1,120 ppb treatment groups. No significant differences were observed in the liver or spleen-to-body weight ratios among the groups. Using high-performance liquid chromatography, the analysis of samples from the treatment groups revealed the absence of DON in both eggs and blood, with very low levels detected in excreta. No DON was detected in the excreta of the control group. Calculating the toxin digestibility rates showed that, in the first week of the experiment, the group fed with 1,120 ppb of DON exhibited significantly higher digestibility rates compared to the 35 and 70 ppb treatment groups (P < 0.05). In conclusion, the long-term consumption of feed containing low-dose DON by laying hens does not significantly impact the safety of the eggs produced or egg production and feed conversion ratios. However, at 40 weeks of age, eggshell strength significantly decreases with a substantial increase in the low-dose DON concentration in the feed. Some minor effects on the health of the chickens were observed, but the magnitude was not significant. |
本系統中英文摘要資訊取自各篇刊載內容。