頁籤選單縮合
題 名 | Characterization of Ca²⁺-sensing Receptor-mediated Ca²⁺ Influx in Microvascular bEND.3 Endothelial Cells |
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作 者 | Leong, Iat-lon; Tsai, Tien-yao; Shiao, Lian-ru; Zhang, Yu-mei; Wong, Kar-lok; Chan, Paul; Leung, Yuk-man; | 書刊名 | The Chinese Journal of Physiology |
卷 期 | 64:2 2021.03-04[民110.03-04] |
頁 次 | 頁80-87+87_1-87_2 |
分類號 | 415.114 |
關鍵詞 | Ca²⁺; Ca²⁺-sensing receptors; Endothelium; Spermine; TRP channels; |
語 文 | 英文(English) |
英文摘要 | Ca2+-sensing receptors(CaSR), activated by elevated concentrations of extracellular Ca2+, have been known to regulate functions of thyroid cells, neurons, and endothelial cells(EC). In this report, we studied CaSR-mediated Ca2+ influx in mouse cerebral microvascular EC (bEND.3 cells). Cytosolic free Ca2+ concentration and Mn2+ influx were measured by fura‑2 microfluorometry. High (3 mM) Ca2+ (CaSR agonist), 3 mM spermine (CaSR agonist), and 10 µM cinacalcet (positive allosteric modulator of CaSR) all triggered Ca2+ influx; however, spermine, unlike high Ca2+ and cinacalcet, did not promote Mn2+ influx and its response was poorly sensitive to SKF 96365, a TRP channel blocker. Consistently, 2-aminoethoxydiphenyl borate and ruthenium red (two other general TRP channel blockers) suppressed Ca2+ influx triggered by cinacalcet and high Ca2+ but not by spermine. Ca2+ influx triggered by high Ca2+, spermine, and cinacalcet was similarly suppressed by A784168, a potent and selective TRPV1 antagonist. Our results suggest that CaSR activation triggered Ca2+ influx via TRPV1 channels; intriguingly, pharmacological, and permeability properties of such Ca2+ influx depended on the stimulating ligands. |
本系統中英文摘要資訊取自各篇刊載內容。