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題名 | 具拮抗能力放線菌菌株之鑑定與防治馬鈴薯瘡痂病效果評估=Identification and Evaluation of Antagonistic Actinobacteria on Controlling Potato Common Scab |
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作者姓名(中文) | 林靜宜; 倪蕙芳; 林慧如; | 書刊名 | 臺灣農業研究 |
卷期 | 69:2 2020.06[民109.06] |
頁次 | 頁122-131 |
分類號 | 434.32 |
關鍵詞 | 馬鈴薯瘡痂病; 鏈黴菌; 生物防治; Potato common scab; Streptomyces spp.; Biologic control; |
語文 | 中文(Chinese) |
中文摘要 | 馬鈴薯瘡痂病為嚴重影響馬鈴薯產量與品質之重要病害之一,目前並無有效之防治藥劑可用,而輪作及抗病品種等耕作防治法效果亦有限。因此,本研究自高雄不同地區之土壤分離純化85株放線菌後,以玻璃紙抗生法於培養基上初步篩選對馬鈴薯瘡痂病菌Streptomyces europaeiscabiei具有拮抗活性之菌株,再利用拮抗菌株之培養濾液進一步測試其拮抗活性。結果發現,菌株35-2與43-21對S. europaeiscabiei病原之拮抗能力最佳。進一步利用16S rRNA基因序列分析後初步鑑定35-2與43-21菌株為鏈黴菌Streptomyces rochei與Streptomyces neopeptinius。溫室試驗結果亦顯示,以菌株35-2與43-21之培養濾液澆灌馬鈴薯植株後,薯塊之罹病度分別為28.0%及35.0%,明顯低於對照組之罹病度61.5%。由此顯示,此兩拮抗菌株具有降低薯塊發生馬鈴薯瘡痂病罹病度之潛力,同時此兩株拮抗菌具有促進馬鈴薯薯塊生長的效果。 |
英文摘要 | Potato common scab is an economically important disease that is caused by several members of the genus Streptomyces in the world. As no effective pesticides and limited results of crop rotation and resistant potato cultivars for managing the disease, this study explored the possibility of using biological control. A total of 85 isolates of actinobacteria were isolated from soil from six different locations in Kaohsiung City. Cellophane paper antibiotic method and bacterial culture filtrates application were conducted to determine antagonistic activity against Streptomyces europaeiscabiei. Isolates 35-2 and 43-21 strongly inhibited S. europaeiscabiei growth in both assays. When potato plants were drenched with culture filtrates of isolates 35-2 and 43-21, respectively, significantly reduced potato common scab incidence was observed in pot assays. The disease severity was 28% after isolate 35-2 treatment and 35% after isolate 43-21 treatment. In addition, isolates 35-2 and 43-21 increased potato tubers weight in pot assays. These results suggested that isolates 35-2 and 43-21 could provide a dual benefit by decreasing potato common scab disease severity and increasing potato yield as an effective biological control agent. Based on 16S rRNA sequencing analysis, the two antagonistic isolates 35-2 and 43-21 showed a sequence identity of 100% and 99% with Streptomyces rochei and Streptomyces neopeptinius, respectively. |
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