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題 名 | 以次世代高通量定序技術探討不同氧化劑對油污染土壤菌群結構之影響=Influences of Different Oxidants on Microbial Community Structure in Petroleum-Hydrocarbon Contaminated Soil |
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作 者 | 陳薇羽; 吳哲宏; 陳宏達; 陳琪璜; 涂茂園; | 書刊名 | 石油季刊 |
卷 期 | 53:1 2017.03[民106.03] |
頁 次 | 頁101-113 |
分類號 | 445.6 |
關鍵詞 | 總石油碳氫化合物; 現地化學氧化; 現地生物復育; 高通量定序; 微生物菌群結構; Petroleum hydrocarbon; In situ chemical oxidation; ISCO; Bioremediation; High-throughput sequencing; Bacterial community structure; |
語 文 | 中文(Chinese) |
中文摘要 | 台灣通過土壤與地下水污染整治法後,有許多場址公告為控制或整治場址。以現地氧化處理高濃度污染區域,再銜接加強式現地生物復育整治土壤中剩餘污染濃度是兼顧處理效率與整治成本的方法。本研究調查分別以過碳酸鈉、過碳酸鈉加亞鐵、雙氧水加氧鐵、過硫酸鈉加亞鐵化學藥劑處理油污染土壤前後的菌群變化,評估現地化學氧化結合現地生物整治油污染土壤的可能性。結果顯示在化學氧化期,總石油碳氫化合物去除率皆低於20%,直至生物整治時期,柴油碳氫化合物的去除率開始上升,其中過碳酸鈉加亞鐵去除率達73.3%。同時,配合高通量定序技術分析土壤中菌群結構的變化,化學藥劑與亞鐵混合會導致Proteobacteria 菌群減少,而誘導出Acidobacteria 、Firmicutes 、Verrucomicrobia 與Actinobacteria 菌群,顯示亞鐵添加或化學氧化反應對微生物族群結構變動影響很大。另一方面,結合主成分分析結果顯示Geothrix 菌群在降解總石油碳氫化合物過程中扮演重要的角色。最後,從高通量定序結果發現注入不同化學藥劑之後,環境中可偵測到許多降解菌群(如:Methylobacterium、Sphingomonas、Rhodococcus、Propionibacterium、Pseudoxanthomonas、Micrococcus、Burkholderias 及Novosphingobium),表示後續只要持續添加適當的營養鹽持續讓土壤微生物生長保持活性,是非常適合進行後續的微生物復育。 |
英文摘要 | In situ chemical oxidation (ISCO) in combination with the bioremediation method is an attractive technology to effectively remedy petroleum-hydrocarbon contaminated soil. This composite technology is involved in the chemical reactions of oxidants to decompose/desorb the hydrocarbons from the soil particles, followed by a microbiological degradation process to mineralize the pollutants. The sophisticated operation of the two processes requires understanding of effects of oxidants on soil microbial community structure. However, the underlying knowledge is very limited. In this study, we analyzed microbial communities before and after three oxidants (sodium percarbonate, hydrogen peroxide and sodium persulfate) were used to remedy petroleum-hydrocarbon contaminated soil and evaluated the influence of low-dosage chemical oxidation on soil microbial community structures. The results showed that during chemical oxidation phase, the three oxidants led to different pH environments with hydrocarbon removal efficiencies lower than 20%. Higher removal efficiencies were observed during the bioremediation phase. Among the three oxidants used, sodium percarbonate mixed with ferrous ion had the highest removal efficiency, 73.3%. The analysis of the V3-V4 region of bacterial 16S rRNA gene showed that the microbial community structures in the bioremediation phase were greatly determined by the oxidants used with or without the ferrous ion. Despite the oxidants, many hydrocarbon-degrading microbial populations, such as Methylobacterium, Sphingomonas, Rhodococcus, Propionibacterium, Pseudoxanthomonas, Micrococcus, Burkholderia and Novosphingobium could be still detected in medium to high abundance. Principal component analysis suggested that the members of the genus Geothrix can survive at the usage of the three oxidants, and played an important role in degrading the hydrocarbons at a broad range of pH conditions. The overall results obtained in this study demonstrated the potential of the composite technology for the remediation of hydrocarbons from the soil. |
本系統中英文摘要資訊取自各篇刊載內容。