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題名 | Using a PCR-DGGE Method to Analyze Diazotrophic Diversity in Soil and Root Nodules of Casuarina in the Sihu Area=利用PCR-DGGE技術分析四湖地區木麻黃林分土壤及根瘤之固氮微生物多樣性 |
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作者姓名(中文) | 張琮柏; 洪俊雄; 吳羽婷; 林瑞進; | 書刊名 | 臺灣林業科學 |
卷期 | 32:2 2017.06[民106.06] |
頁次 | 頁101-110 |
分類號 | 436.193 |
關鍵詞 | 木賊葉木麻黃; 變性梯度膠體電泳; 固氮菌多樣性; NifH基因; 根瘤; Casuarina equisetifolia; Denaturing gradient gel electrophoresis; DGGE; Diazotrophic diversity; Frankia; NifH gene; Root nodule; |
語文 | 英文(English) |
中文摘要 | 木麻黃是臺灣沿海地區主要之防風樹種,其具有生長快速及廣泛的逆境適應能力,可提供土壤穩定、沿海保護及棲地復育之功能。在臺灣西部沿海木麻黃防風林遭遇到許多天然更新之限制因子,例如:土壤貧瘠、乾旱、鹽分逆境、枯落物及雜草競爭。然而,氮元素通常是植物生長主要的限制因子之一,木麻黃植物可以透過與固氮菌Frankia共生形成根瘤,藉此,獲得氮源。為了瞭解不同木麻黃林分與固氮微生物多樣性之交互關係,健康的木麻黃林分與衰敗的林分相較,是否具有較高的固氮微生物多樣性,以及木麻黃根瘤內具有多少株固氮微生物。本研究藉由引子polF-GC/polR直接擴增nifH固氮基因,並進行變性梯度膠體電泳分析,以了解土壤中固氮微生物之多樣性。研究結果顯示木麻黃根瘤中應具有2群團之固氮微生物,而不同木麻黃林分土壤中具有高度的固氮微生物群落多樣性,無論林分衰敗與否。因此,本研究證實木麻黃林分之衰敗與固氮微生物多樣性無直接的關係。 |
英文摘要 | Casuarina spp. are major windbreak species in coastal forests of Taiwan. With great ability for rapid growth and adaptability to adverse environmental conditions, they enhance soil stabilization and provide protection and rehabilitation for coastal areas. Many factors may limit the reproduction of Casuarina in coastal areas such as infertile soil, drought, salt stress, litterfall, and weeds. In addition, nitrogen is generally considered one of the major nutrients in plant growth, and Casuarina plants can obtain nitrogen sources through association with Frankia via root nodules. In order to understand the interaction between diverse diazotrophic diversity and different test sites of Casuarina spp. determine whether it is the diversity level of diazotrophic on healthy and on declining Casuarina spp., or the number of nitrogen-fixing bacteria in the nodules of Casuarina spp., direct amplification of the nifH gene for a polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis with the primer pair polF-GC/polR was applied in this study. The results demonstrated that there were 2 groups of nitrogen-fixing bacteria collected from these root nodule samples that appeared on the PCR-DGGE profiles. Whether Casuarina stands are on the decline or not, a high diversity of diazotrophic community has been observed among all test sites. Therefore, this study shows that the declining Casuarina is not related to the diazotophic diversity. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。