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題 名 | Enzymes-hydrolyzed Fucooligosaccharides from Sargassum siliquosum and Their Intestinal Transportation Across Human Caco-2 Cells=自莢托馬尾藻(Sargassum siliquosum)以混合酵素生產岩藻寡醣並於人類腸癌細胞株Caco-2測試吸收及運輸之變化 |
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作 者 | 張劭強; 李樵; 陳柏璇; 卡伯特; 張君如; 潘崇良; | 書刊名 | 臺灣水產學會刊 |
卷 期 | 42:1 2015.03[民104.03] |
頁 次 | 頁25-37 |
分類號 | 379.25 |
關鍵詞 | 莢托馬尾藻; 岩藻寡醣; Caco-2細胞株; 腸道吸收與運輸; Sargassum siliquosum; Fucooligosaccharide; FOS; Caco-2 cell; Intestinal absorption and transport; |
語 文 | 英文(English) |
中文摘要 | 岩藻寡醣為褐藻中主要分之一,其具有抗氧化、抗凝血、與抗病毒之生理活性。本研究目的為探討自莢托馬尾藻(Sargassum siliquosum)生產岩藻寡醣,並於人類結腸癌細胞株Caco-2之腸道模式下,評估岩藻寡醣於腸道吸收及運輸之變化。自莢托馬尾藻萃取岩藻聚醣,同時添加Fucosidase rfuc I-H、MA103與MAEF108粗酵素液水解24 hr水解岩藻聚醣所得岩藻寡醣水解液利用超過濾系統區分出小於3 kDa分子量之寡醣,經過Sephadex G-10膠體過濾層析(Gel permeation chromartography, GPC)可獲得岩藻混合寡醣Fuc-S1與Fuc-S2。利用高效能液相層析儀(High performance liquidchromatograph, HPLC)分析結果顯示Fuc-S1與Fuc-S2之滯留時間分別為19-24 min與19-23 min,初步推估Fuc-S1與Fuc-S2之聚合度為DP 2-10與DP 2-8。岩藻混合寡醣Fuc-S1與Fuc-S2於Caco-2細胞吸收運輸的結果顯示上層液中岩藻混合寡醣Fuc-S1之含量從3.71 ± 0.73 mg/ml降至3.04 ± 0.34 mg/ml,下層液中含量則從2.33 ± 0.31 mg/ml上升至2.80 ± 0.53 mg/ml,而被Caco-2細胞吸收之含量相似約在4.07 ± 0.60 mg/ml。岩藻混合寡醣Fuc-S2之上層液岩藻寡醣含量自2.93 ± 0.24 mg/ml至2.14 ± 0.86 mg/ml,下層液則從2.15 ± 0.18 mg/ml至3.10 ± 0.27 mg/ml,而被Caco-2細胞吸收之含量約4.48 ± 0.80 mg/ml。於腸道細胞Caco-2測試系統中反應90 min時,即岩藻混合寡醣Fuc-S1之運輸率約為28.1%,細胞吸收率約為39.0%;而岩藻混合寡醣Fuc-S2之運輸率約為31%,細胞吸收率約為47.6%。 |
英文摘要 | Fucooligosaccharide is one of the major active components in brown algae. Fucooligosaccharide (FOS) possesses antioxidative, anti-coagulation and antiviral effects, but its intestinal bioavailability needs further evaluation. This study aims to produce FOS from S. siliquosum and to determine their absorption and transportation in human enterocytes. Exploring the absorption and transportation of the Fuc-S1 and Fuc-S2 mixture by using the in vitro Caco-2 monolayers confirmed that the concentration of Fuc-S1 in the apical fluid decreased from 3.71 ± 0.73 mg/ml to 3.04 ± 0.34 mg/ml, whereas the concentration in the basolateral fluid increased from 2.33 ± 0.31 mg/ml to 2.80 ± 0.53 mg/ml. Therefore, the amount of Fuc-S1 absorbed by the Caco-2 monolayers was approximately 4.07 ± 0.60 mg/ml. Furthermore, the concentration of Fuc-S2 in the upper layer solution decreased from 2.93 ± 0.24 mg/ml to 2.14 ± 0.86 mg/ml, whereas the concentration in the lower layer solution increased from 2.15 ± 0.18 mg/ml to 3.10 ± 0.27 mg/ml. Accordingly, the amount of Fuc-S2 absorbed by the Caco-2 monolayers was approximately 4.48 ± 0.80 mg/ml. Loading Fuc-S1 and Fuc-S2 into an intestinal Caco-2 system to test the associated reactions for 90 min revealed that the transportation and cellularabsorption rates of Fuc-S1 were 28.1% and 39.0%, respectively, whereas those of Fuc-S2 were 31% and 47.6%, respectively. |
本系統中英文摘要資訊取自各篇刊載內容。