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題 名 | 孕婦產前檢查乙型鏈球菌CMP™ GBS Carrot Broth與CMP™ GBS TransCultSwab的效能評估=The Performance of GBS Carrot Broth and GBS TransCultSwab in the Detection of Group B Streptococci in Near-term Pregnant Women |
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作 者 | 蔡偉勳; 何承蓉; 洪晟峰; 蔡文城; | 書刊名 | 檢驗及品保雜誌 |
卷 期 | 5:1 2016.02[民105.02] |
頁 次 | 頁1-10 |
分類號 | 417.14 |
關鍵詞 | 孕婦產前檢查乙型鏈球菌; 乙型鏈球菌增菌培養基; CMP™ GBS carrot broth; CMP™ GBS TransCultSwab; GBS carrot broth; GBS TransCultSwab; Group B streptococci; GBS; Performance; β/γ GBS detection agar/GBS carrot agar; |
語 文 | 中文(Chinese) |
中文摘要 | CMPTM GBS carrot broth與CMPTM GBS TransCultSwab為啟新生物科技有限公司(新北市,台灣)針對孕婦產前檢查乙型鏈球菌(B群鏈球菌,Group B Streptococcus, Streptococcus agalactiae, GBS)的創新設計,兩者均宣稱可用於GBS的增菌與鑑別(陽性呈現胡蘿蔔色),而後者更具有採檢與輸送的功能。為了確認CMPTM GBS carrot broth與CMPTM GBS TransCultSwab的效能,吾等首先進行GBS偵測極限測定,結果發現兩者對GBS的偵測極限皆為10 CFU。另以54株生殖道常在菌及臨床致病菌進行特異性分析,結果顯示除了溶血型GBS外的其它菌種皆不顯色,因此,兩者的特異性均為100%。進一步將這些測試菌與溶血型GBS混合後分別接種CMPTM GBS carrot broth 與GBS TransCultSwab,結果發現高倍數菌量(100x)的Enterococcus spp.(腸球菌)與溶血型(不溶血型)GBS 會分別干擾溶血型GBS在二種增菌培養基的顯色,其它菌種無論高倍或相等菌量混合皆無影響。另外,與Lim broth增菌效能相較結果指出三種培養基並無顯著差異。基於上述的發現,吾等認為CMPTM GBS carrot broth 與CMPTM GBSTransCultSwab皆適用於孕婦產前GBS檢查,更因為GBS增菌會促進顯色而可做為GBS鑑別的依據。兩種增菌培養基與/ GBS Detection Agar與GBS Carrot Agar bi-plate的配合應用將可簡化GBS檢測的操作流程、縮短報告時間以及降低操作人力,係檢測GBS的有效工具。 |
英文摘要 | The GBS carrot broth and GBS TransCultSwab are innovative media used for the enrichment and screening for Group B streptococci (GBS) in near-term pregnant women (at 35~37 weeks of gestation). Creative Microbiologicals, Taiwan claims these media are excellent for enrichment and identification, furthermore, the latter were even with specimen’s collection and transport. To verify the performance of both media, we first evaluated their detection limits for GBS and found both were 10 CFU. We then employed 54 species/strains of microorganisms that were frequently encountered clinically in the birth canal or in other sites to evaluate the specificity of both media. The results showed that the presence of the GBS ß -hemolytic strain turns either the GBS carrot broth or the GBS Trans CultSwab into an orange color, which does not Trans- occur with the other test species/strains. Therefore, the GBS specificity of both media was 100%. Interference studies were evaluated by mixing GBS (ß -hemolytic) with one of the 54 test species/strains. The results indicated that although the presence of a large amount (100x) of Enterococcus spp. and GBS (ß -hemolytic) interferes with the carrot color production of both media, similar amount of the remaining test species/strains do not. In addition, we compared the enrichment capability of GBS carrot broth and GBS TransCultSwab with that of Lim broth and found there were no significant differences among them. In summary, if either GBS carrot broth or GBS TransCultSwab is combining with/ GBS detection agar/GBS carrot agar subculture during the culture process in a prenatal GBS examination, this could simplify the laboratory procedure, accelerate GBS identification, shorten the final report, and reduce time spent on the procedure. Therefore, both media are useful as an inoculation culture medium for the detection of GBS in the laboratory. |
本系統中英文摘要資訊取自各篇刊載內容。