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題 名 | Mycobacteria Growth Indicator Tube (MGIT)的污染率及汙染菌的類別與藥敏型式=The Contamination Rates and Types of Contaminants and Antibiograms Found When Mycobacteria Growth Indicator Tubes Are Used in a Mycobacterial Laboratory |
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作 者 | 洪婷; 蔡偉勳; 吳佩真; 蔡岳廷; 蔡文城; 洪婷; | 書刊名 | 檢驗及品保雜誌 |
卷 期 | 4:4 2015.11[民104.11] |
頁 次 | 頁165-172 |
分類號 | 414.83 |
關鍵詞 | MGIT液體培養管; 污染菌類別; 常見的污染菌; 藥敏試驗; MGIT contaminants; PANTA; Antibiograms; |
語 文 | 中文(Chinese) |
中文摘要 | 當前台灣檢測分枝桿菌的檢驗室均使用Mycobacteria Growth Indicator Tube (MGIT)液體培養管配合自動化的BACTEC MGIT 960 system,以便提早發出報告。雖然MGIT 管添加5 種抗微生物劑(polymyxinB、amphotericin B、nalidixic acid、trimethoprim 與azlocillin,簡稱PANTA)抑制檢體中分枝桿菌除外的各種棲息菌,但檢驗室仍受到高污染率(1.9~30%,通常10%以上)的困擾,高污染率將降低分枝桿菌(MTBC及NTM)的分離率以及增加檢驗室處理污染MGIT 管的人力及物力,因此降低MGIT 污染率有其重要性。為了達到降低污染率的目標,首先需先了解MGIT 管的污染菌類別。本研究以MGIT 陽性,螢光染色陰性的MGIT管移種BAP/EMB以分離常見的污染菌,首先評估發現的303 種污染菌,發現主要類別為腸內菌、葡萄糖非發酵性革蘭氏陰性桿菌、芽孢桿菌(Bacillus spp.)與葡萄球菌(staphylococci),其等分別佔42.9%、30.0%、15.8%與11.2%。利用瓊脂紙錠擴散試驗方法操作腸內菌及葡萄糖非發酵性革蘭氏陰性桿菌對臨床常用藥物的藥敏試驗,結果指出此兩類菌對ceftriaxone、imipenem、ciprofloxacin 與gentamicin 分別呈現不同程度的感受性,而Bacillus spp.對vancomycin 呈100%感受性。另外,以瓊脂稀釋方法進行分離菌中S. aureus 的藥敏試驗,由vancomycin 的最低抑菌濃度(MIC)結果指出S. aureus 對vancomycin 呈100% 感受性。基於上述的發現以及vancomycin不會影響分枝桿菌的生長,吾等建議台灣地區的分枝桿菌檢驗室可選擇適當濃度的vancomycin 連同PANTA 加到MGIT 管,將具有降低革蘭氏陽性球菌及Bacillus spp.污染的潛力。另外,亦可根據MGIT 管常見汙染菌的類別評估其等對PANTA 的最低抑菌濃度(MIC),以為調整PANTA 中部份抗生素濃度的依據。 |
英文摘要 | At present, all mycobacterial laboratories use Mycobacteria Growth Indicator Tube (MGIT) liquid medium combined with the automatic BACTEC MGIT 960 system for the early reporting of mycobacteria. Although MGIT is incorporated with 5 kinds of antimicrobial agents (namely polymyxin B, amphotericin B, nalidixic acid, trimethoprim, and azlocillin, which are collectively abbreviated as PANTA) to inhibit all kinds of contaminants other than mycobacterial species, many laboratories still suffer from high contamination rates (1.9~30%, with the average being above 10%) which result in decreased isolation rates of both Mycobacterium tuberculosis complex (MTBC) and nontuberculous mycobacteria (NTM), in addition to increasing the use of laboratory labor and resources. For this reason, it would be extremely valuable to lower the contamination rates associated with the MGIT system. In pursuing this goal of decreased contamination, however, it is important to first understand the kinds of contaminants present in MGIT. To do so, we transfered positive MGIT, which was negative in a fluorescent smear, to a BAP/EMB bi-plate to isolate the contaminants frequently encountered in laboratories. We evaluated 303 strains of contaminants and found that the major categories were Enterobacteriaceae, glucose non-fermenting Gramnegative bacilli (GNF), Bacillus spp. and staphylococci, and that their isolation rates were 42.9%, 30.0%, 15.8%, and 11.2%, respectively. We also used the agar disk diffusion method to conduct antimicrobial susceptibility testing for Enterobacteriaceae and GNF, and the results indicated different degrees of susceptibility for these two categories of bacteria to ceftriaxone, imipenem, ciprofloxacin, and gentamicin, whereas Bacillus spp. were l00% susceptible to vancomycin. Additionally, the agar dilutions we employed were l00% susceptible to vancomycin. The agar dilution method was employed to determine the minimal inhibitory concentration (MIC) of S. aureus isolates to vancomycin and found they were 100% susceptible to vancomycin. Based on the above findings and the fact that mycobacterial growth will not be affected by vancomycin, we recommend that the appropriate concentration of vancomycin may be selected for combination with PANTA and incorporated into MGIT. This may have the potential to decrease the contamination of MGIT by Grampositive cocci and Bacillus spp. Relatedly, the determination of the MICs of PANTA to the commonly found contaminants may be used as the basis for adjusting the concentrations of some antimicrobials among PANTA. |
本系統中英文摘要資訊取自各篇刊載內容。