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頁籤選單縮合
題名 | 應用ISSR分析蝴蝶蘭屬(Phalaenopsis)物種之遺傳歧異度=The Use of ISSR Marker for Genetic Diversity among Phalaenopsis Species |
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作者 | 沈雅鈞; 胡澤寬; 古新梅; | 書刊名 | 桃園區農業改良場研究彙報 |
卷期 | 76 2014.12[民103.12] |
頁次 | 頁27-42 |
分類號 | 435.432 |
關鍵詞 | 蝴蝶蘭屬; ISSR分子標誌; 遺傳歧異度; Phalaenopsis; ISSR; Molecular markers; Genetic diversity; |
語文 | 中文(Chinese) |
中文摘要 | 本研究旨在探討蝴蝶蘭屬(Phalaenopsis)物種間之遺傳歧異度,針對43 個蝴蝶 蘭屬物種,以及外群臺灣香蘭(Haraella retrocalla (Hayata) Kudô)和名護蘭(Sedirea japonica ( L. Linden & Rchb. f. ) Garay & H.R. Sweet)為材料,以12 個ISSR(intersimple sequent repeat)分子標誌進行分析。結果共增幅出270 個清晰且再現性良好之條 帶,多型性比例占100%,其中以UBC834 產生29 個條帶為最多。PIC(polymorphism information content)與Rp(resolving power)的平均值分別為0.3053 及9.8060。Dice’s coefficient 估算其遺傳相似度,再以UPGMA(unweighted pair group method with arithmetic mean)進行群聚分析,在相似度約0.42 處可將物種區分成兩大群,約0.44 處再分成第一與二類群,約0.46 處再分為第三與四類群。本研究以ISSR 分子標誌分 析結果顯示蝴蝶蘭屬內物種間具有相當之遺傳歧異度,分群結果可說明物種間的親緣 關係遠近,可提供蝴蝶蘭育種者在分子遺傳與輔助育種等相關工作之參考。 |
英文摘要 | The aim of this research is to study the genetic diversity of Phalaenopsis species, including 43 Phalaenopsis species and the outgroup including Haraella retrocalla (Hayata) Kudô and Sedirea japonica (L. Linden & Rchb. f.) Garay & H.R. Sweet using 12 ISSR markers. A total of 270 clear and reproducible bands were amplified and 29 clear bands were amplified by ISSR markers of UBC834, which gave the highest number of bands per marker. The percentage of polymorphism (PIC) and resolving power (Rp), on average, were 0.3053 and 9.8060, respectively. A dendrogram based on Dice’s coefficient was constructed by UPGMA and Phalaenopsis species were clustered into two major groups at the similarity of 0.42, at the similarity of 0.44 distributed group 1 and 2, the similarity of 0.46 distributed group 3 and 4. This study showed certain degree of genetic diversity existed among Phalaenopsis species using ISSR marker analysis, the result of clustering may indicate genetic relationship between species distance, provided breeders the references of molecular genetic and maeker-assisted breeding. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。