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題 名 | 臺灣山葵上發生的山葵斑駁病毒之鑑定=Identification of Wasabi mottle virus Infecting Wasabi in Taiwan |
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作 者 | 鄧汀欽; 蔡錦慧; 寧方俞; | 書刊名 | 臺灣農業研究 |
卷 期 | 65:1 2016.03[民105.03] |
頁 次 | 頁103-108 |
分類號 | 434.94 |
關鍵詞 | 菸草嵌紋病毒屬; 阿里山; 番茄嵌紋病毒; 十字花科; Tobamovirus; Alishan; Tomato mosaic virus; Brassicaceae; |
語 文 | 中文(Chinese) |
中文摘要 | 鄧汀欽、蔡錦慧、寧方俞。2016。台灣山葵上發生的山葵斑駁病毒之鑑定。台灣農業研究65(1):103–108。2012年4月自嘉義縣阿里山鄉採集山葵植株,以葉片汁液進行接種試驗,在奎藜(Chenopodiumquinoa)葉片形成局部病斑,經單斑分離得病毒純系。經酶聯抗體免疫分析(ELISA)及西方轉漬反應(Western blot),這些分離株與番茄嵌紋病毒(Tomato mosaic virus; ToMV)抗體有交互反應 (cross reaction)。接種試驗結果顯示,其寄主範圍主要在十字花科植物,但不感染油菜及初秋甘藍。取其全量RNA,以Tobamovirus廣效性引子對TMVmpf及TMV3nr進行反轉錄-聚合酶鏈鎖反應(RT-PCR),結果得到一個688bp的片段。此片段為病毒的部分鞘蛋白(CP)及部分移動蛋白(MP)的基因,經解序後與GenBank中核苷酸序列比對,結果與山葵斑駁病毒栃木分離株(Wasabi mottle virus Tochigi isolate, Acc. No. AB017504)的對應片段,相同度達99%。親緣分析結果,則顯示其CP基因的產物與Acc No. BAA28951(a wasabi strain of crucifer Tobamovirus)的對應片段之相同度為99.3%,但與ToMV昆士蘭分離株(Acc No. NP_78449)的相同度僅49%,因此鑑定這些山葵分離株即是山葵斑駁病毒 (WMoV)。利用ELISA 檢測阿里山區的山葵植株,共調查187個樣本,其中有66 個呈陽性反應,進一步核酸分析鑑定亦皆為WMoV。本報告雖是台灣WMoV的首發紀錄,但WMoV應早已存在於阿里山區。 |
英文摘要 | Deng, T. C., C. H. Tsai, and F. Y. Ning. 2016. Identification of Wasabi mottle virus infecting wasabi in Taiwan. J. Taiwan Agric. Res. 65(1):103–108. Virus-liked wasabi plant samples were collected in Alishan Township, Chiayi in April 2012. By leaf sap inoculation, local lesions were developed on Chenopodium quinoa, from which pure virus isolates were obtained through single lesion isolation. The isolates were crossly reacted with antibody to Tomato mosaic virus (ToMV) when tested by enzyme-linked antibody immunoassay (ELISA) or Western blot. Results of inoculation tests showed that the host range was mainly limited in cruciferous plants, except for Brassica napus and B. oleracea L. var. capitata cv. Chu-chiu. Total viral RNA was purified and a pair of Tobamovirus-general primers (TMVmpf and TMV3nr) was applied to conduct reverse transcription polymerase chain reaction (RT-PCR), and that resulted in producing a 688 bp DNA fragment. The nucleotide sequence of the fragment (part of the CP gene and part of MP gene) was determined. BLAST analysis of this fragment revealed the virus isolate shared the maximum nucleotide identities (99%) with Wasabi mottle virus Tochigi isolate (AB017504). Phylogenetic analysis also showed that the viral CP gene was 99.3% identical to a wasabi strain of crucifer Tobamovirus (Acc No. BAA28951), compared with 49% identities to an isolate of ToMV from Queensland, Australia (NP_078449). Therefore, the virus isolates were identified as a Wasabi mottle virus (WMoV). However, the virus was detected in 66 out of 187 wasabi plant samples collected in Alishan area and all of these positive samples were further identified as WMoV by nucleic acid analysis. This paper is the first report of WMoV occurred in Taiwan, but WMoV should have been existed in the Alishan area already. |
本系統中英文摘要資訊取自各篇刊載內容。