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| 題 名 | 中草藥應用於保健食品開發之安全性研究=Safety Evaluation on the Chinese Herbal Medicine for Health Food Development |
|---|---|
| 作 者 | 劉崇喜; | 書刊名 | 中醫藥年報 |
| 卷 期 | 3 2014.12[民103.12] |
| 頁 次 | 頁(48)0-(48)56 |
| 分類號 | 414.34 |
| 關鍵詞 | 生薑; 刺五加; 薏苡; 安全性; 基因毒性; Zingiber officinale Roscoe; Acanthopanax senticosus; Coix lachryma-jobi L; Safety; Genetic toxicity; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 研究目的:本計畫利用臺灣產生薑 (Zingiber officinale Roscoe)、刺五加 (Acanthopanax senticosus)及薏苡 (Coix lachryma-jobi L.)三種藥材組成有效抑制脂肪細胞增生之最適配方,期能日後應用於「不易形成體脂肪功能」之健康食品安全性劑量的參考。 研究方法:本研究分別測定藥材臺灣產生薑 (Zingiber officinale Roscoe)、刺五加 (Acanthopanax senticosus)及薏苡 (Coix lachryma-jobi L.)進行重金屬和農藥殘留並檢測藥材指標成份含量。並以不同炮炙方法及溶媒萃取進行最佳配方比例篩選製造成膠囊劑型。本研究運用 3T3-L1細胞針對研究中不同藥材比例配方探討脂肪細胞增生及分化率測定之影響,將待測中草藥膠囊劑型進行健康食品法所規範之安全性測試:急性毒性試驗、 28天餵食毒性試驗及基因毒性試驗。 結果與討論:本計畫使用之待測中藥材無農藥殘留而重金屬含量都在合格範圍內。由指標成分含量的變化結果顯示臺灣生薑炮炙後的生薑 [6]-薑辣醇([6]-gingerol)含量比生薑少,而大陸乾薑文火炮炙後能提升 [6]-薑辣醇含量。醋炙臺灣產或是大陸產之刺五加其紫丁香苷 (syringin)含量皆明顯增加,但刺五加苷 (eletheroside)含量減低。刺五加、生薑、紅薏仁的炮製品以 8:1:1的比例混合並不會抑制 3T3-L1細胞的增生,但抑制胞內脂質蓄積的效果最佳。將此複合物投與 BALB/cByJNarl 雄性小鼠進行急性毒性試驗,其半數致死劑量 (LD50)皆大於 5 g/kg,顯示此藥材及其炮製品之口服急性毒性甚低,連續餵食 28天生化分析與病理切片結果皆屬正常,基因毒性試驗、微核試驗及沙門菌回復突變試驗檢測結果亦無任何突變現象。 |
| 英文摘要 | AIM: The aim of the study were on the establishment of a prescription composed of Zingiber officinale Roscoe,Acanthopanax senticosus and Coix lachryma-jobi L. for body weight control. These tests will provide referencing data for the setting up of safety dosages that will later be used in subsequent experiments on the efficacy of health food products aimed at controlling of adipocyte formation. METHOD: In addition to analyze the contents of active compounds, the heavy metal and chemicals present on the tree targeted herbs were estimated. The inhibition of prescription on growth and differentiation of 3T3-L1 preadipocytes was estimated. The experimental tests on the safety and stability of tested prescriptions were also conducted, including acute and subchronic (28 days) toxicity tests, as well as genetic toxicity test. RESULTS & DISCUSSION: There was no insecticides exists in the tested herbs, in addition, the metal contents in the tested herbs were under qualified scope. The content of [6]-gingerol on the soft-fire proceed Z officinale cultured in Taiwan was lower than that from the raw herb. However, the content of [6]-gingerol in Z officinale cultured in China was increased after processing with gentle heat. The contents of syringin in A senticosus cultured in Taiwan or China were reduced after vinegar-processing; in contrast, the contents of eletheroside in both vinegar-proceeded products were elevated as compared to their raw material. The prescription composed of the proceeding products with Z officinale, A senticosus and C lachryma-jobi at the ratio of 8:1:1 pose the best ability to inhibit lipid accumulation in 3T3-L1 adipocyte without cytotoxicity. In the acute toxicity test, the targeted prescription did not produce any toxic signs or deaths; the 50% lethal dose must be higher than 5 g/kg. Neither subchronic toxicity nor genetic toxic effects was observed in the targeted prescription. Hence, the prescription composed of the proceeding products with Z officinale, A senticosus and C lachryma-jobi at the ratio of 8:1:1 is with the capacity for the development of products applied for lowering body fat formation, or plasma lipids regulation. |
本系統中英文摘要資訊取自各篇刊載內容。