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| 題 名 | 比較初期或延緩之低功率雷射光治療對於神經導管接合老鼠截斷大缺陷坐骨神經之神經再生影響評估=Comparison of Neural Regeneration in a Nerve Conduit across a Large Gap of the Transected Sciatic Nerve in Rats with Early or Delayed Therapeutic Modalities of a Low-Level Laser Phototherapy |
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| 作 者 | 周君柔; 楊怡津; 劉百栓; | 書刊名 | 中華放射線技術學雜誌 |
| 卷 期 | 39:4 2015.12[民104.12] |
| 頁 次 | 頁187-196 |
| 分類號 | 416.293 |
| 關鍵詞 | 周邊神經損傷; 神經導管; 低功率雷射; 坐骨神經修復; Peripheral nerve injury; Nerve conduit; Low-level laser; Sciatic nerve repair; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 本研究以綠梔子素(Genipin)交聯幾丁聚醣(Chitosan)並添加奈米碳管(Carbon nanotube)製備而成的可降解性神經導管(簡稱GCC)。利用大鼠周邊神經斷裂損傷模式,將該神經導管接合大鼠兩斷端坐骨神經,於手術隔天進行不同治療模式的低功率雷射光(low-level laser, LLL)照射輔助治療,比較初期治療組(Laser/Early)或延緩治療組(Laser/Delay)之低功率雷射光治療對於神經導管接合老鼠截斷大缺陷坐骨神經之神經再生影響評估。將實驗動物分為四個組別,第一組為未接收雷射光治療控制組(Laser/Sham);第二組為手術後隔天接受每天2分鐘雷射光初期治療組(Laser/Early);第三組為手術後延遲7天接受每天2分鐘雷射光延緩治療組(Laser/Delay);第四組為自體神經移植組(Autograft)。在坐骨神經功能指數(Sciatic functional index, SFI)分析方面,雷射光治療組(Laser/Early 與Laser/Delay)與Autograft組於每個實驗時期皆顯示高於Laser/Sham組;於術後第12週時,顯示Laser/Delay組的SFI與Autograft組相近,然而Laser/Early組的SFI則稍優於上述兩組。然而,在腓腸肌萎縮評估結果則顯示Laser/Early組比Laser/Delay組(P< 0.05)及Autograft組更具減緩腓腸肌肉萎縮的作用。此外,在再生神經組織免疫染色結果,顯示出低功率雷射光治療可以刺激受損神經的再生修復,且Laser/Early組具有更佳的修復效果。綜合實驗結果顯示在神經受損初期就接受雷射光治療比神經受損延緩治療具有更佳的修復療效。 |
| 英文摘要 | This study used a newly developed nerve guidance conduit made using genipin-cross-linked chitosan and carbon nanotubes (GCC nerve guide conduit). The aim of this study was to analyse the influence of low-level laser on the neurorehabilitation of transected sciatic nerves after bridging the GCC nerve guide conduit in rats. Immediate and deferred treatment models were compared to investigate the impact on impaired sciatic nerve regeneration in rats. The rats were divided into four groups: the Laser/Sham group (no laser therapy), the Laser/Early group (two minutes of laser therapy per day, started from the day of surgery and lasted for 14 days), the Laser/Delay group (two minutes of laser therapy per day, started from the seventh day of surgery and lasted for 14 days), and the Autograft group. Each group consisted of six rats for a total of 24 rats.Firstly, twelve weeks after surgery, our results indicated that the groups who had underwent laser therapy showed significantly improved sciatic nerve function compared to the groups who had no laser therapy and the Laser/Early group showed more improvement than the Laser/Delay group. The Laser/Early group also exhibited less gastrocnemius atrophy than the Laser/Delay group (P< 0.05)and the Autograft group. In addition, the immunohistochemical results of nerve regeneration indicated that low-level laser therapy can stimulate damaged nerve repair and regeneration; also the Laser/Early group showed superior outcomes. In summary of the experimental results above, laser therapy administered immediately after nerve damage has better treatment efficacy over delayed laser therapy. |
本系統中英文摘要資訊取自各篇刊載內容。