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題名 | 蝙蝠蛾擬青霉(Paecilomyces hepiali)菌絲體發酵液之基因毒性及急性毒性探討=Evaluation of Acute Toxicity and Genotoxic Activity of Paecilomyces hepiali in Submerged Culture |
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作者 | 吳思穎; 葉淑幸; 蔡岳廷; 洪鳴遠; 高均瑋; 郭家芬; 陳勁初; Wu, Szu-yin; Yeh, Shu-hsing; Tsai, Yueh-ting; Hung, Ming-yuan; Kao, Chun-wei; Kuo, Chia-feng; Chen, Chin-chu; |
期刊 | 檢驗及品保雜誌 |
出版日期 | 20150500 |
卷期 | 4:2 2015.05[民104.05] |
頁次 | 頁67-77 |
分類號 | 412.36 |
語文 | chi |
關鍵詞 | 蝙蝠蛾擬青霉; 冬蟲夏草; 基因毒性試驗; 急性毒性試驗; Paecilomyces hepiali; Ophiocordyceps sinensis; Genotoxicity; Acute toxicity; |
中文摘要 | 蝙蝠蛾擬青霉(Paecilomyces hepiali) 為中藥材冬蟲夏草[Ophiocordyceps sinensis (Berk.)] 中分離出之伴生菌,根據研究顯示其具有諸多生理活性,因此可作為優良的保健食品素材。本研究使用蝙蝠蛾擬青霉菌絲體發酵液凍乾粉作為試驗物質,進行三項基因毒性及小鼠口服急性毒性試驗,以評估其安全性。結果顯示,於沙門氏菌回復突變試驗、體外哺乳類細胞染色體結構異常試驗及囓齒類週邊血液微核試驗基因毒性試驗中,分別在劑量5.0 mg/plate、5.0 mg/mL及5.0 g/kg•bw以下,皆未發現與試驗物質相關之異常;而單一劑量口服急性毒性試驗也未觀察到血清生化值異常以及任何臨床病變,顯示其LD50 大於12 g/kg•bw。綜合上述,我們判斷蝙蝠蛾擬青霉菌絲體發酵液凍乾粉並無基因毒性及口服急性毒性之疑慮,此結果可作為人類食用安全性之參考。 |
英文摘要 | Paecilomyces hepiali, isolated from natural [Ophiocordyceps sinensis (Berk.)], is known to possess several functional activities and hence has potential beneficial use in healthcare. To ensure more information about the safety of Paecilomyces hepiali whole broth, the standard battery of genotoxicity tests and acute oral toxicity were performed. In results, Paecilomyces hepiali whole broth showed no mutagenicity up to 5mg/plate when tested with five Salmonella typhimurium strains with or without metabolic activation. Similarly, in vitro chromosomal aberration assay did not reveal any significant alterations up to 5 mg/mL as compared to the negative control both in the presence and absence of the metabolic activation (S9 mix). Moreover, the Paecilomyces hepiali whole broth with doses up to 5.0 g/kg.bw, did not increase the incidence of micronucleated polychromatic erythrocytes in mouse bone marrow. Lastly, oral administration of Paecilomyces hepiali whole broth resulted in no mortalities or evidence of adverse effects and the lethal dosage (LD50) was greater than 12 g/kg.bw. Based on the above observations, it can be concluded that Paecilomyces hepialid whole broth is not mutagenic, is generally nontoxic on acute oral administration and should not be considered as a potential concern for humans. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。