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題 名 | 地黃無菌播種及微體繁殖研究=In Vitro Seed Germination and Micropropagation of Rehmannia glutinosa Libosch |
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作 者 | 曹進義; 陳威臣; 吳姿穎; 夏奇鈮; | 書刊名 | 臺灣農業研究 |
卷 期 | 64:3 2015.09[民104.09] |
頁 次 | 頁177-188 |
分類號 | 434.92 |
關鍵詞 | 地黃; 微體繁殖; 無菌播種; 芽體誘導; Rehmannia glutinosa Libosch; Micropropagation; In vitro seed germination; Shoot induction; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究利用地黃(Rehmannia glutinosa Libosch)種子進行無菌播種,地黃種子以0.6%次氯酸鈉溶液消毒15 min可獲得良好之殺菌效果,可明顯降低汙染率,並有最高發芽率,分別為5%與80%。以無菌播種地黃小苗之葉片與葉柄作為微體繁殖材料,接種於含有3%蔗糖、0.8% Bacto agar及0.01 mg L-1 NAA之MS培養基(以下簡稱基本培養基)並添加0-2.0 mg L-1 BA不同濃度,分別於光照與黑暗環境下培養。結果顯示,暗處理下培植體有較高之成活率,且葉柄培植體較葉片培植體之再生反應為佳,葉柄培植體於含有2.0 mg L-1 BA培養基中於黑暗環境下培養具有最高之存活率62%,可同時誘導出芽體與癒傷組織,分別為10%與62%。另以地黃組培苗之頂芽莖段與莖中段2種不同莖段部位作為微體繁殖材料,接種於添加不同蔗糖濃度(3-9%)之基本培養基中培養,結果顯示以3%蔗糖為最佳,成活率可達100%,9%高蔗糖濃度為最差,會導致培植體褐化死亡。進一步利用組培苗之頂芽莖段、莖中段與近基部莖段3 種不同莖段部位培植體培養於含有3%蔗糖濃度並添加0-2.0 mg L-1 BA濃度之基本培養基中誘導芽體,3 種培植體中以頂芽莖段表現最佳,成活率為80-100%,BA濃度於0.5-1.0 mg L-1之間可誘導出最多芽體,每1培植體平均可誘導出27個芽。莖段培植體與BA濃度二者皆顯著影響地黃芽體之誘導率與培養存活率,且二者間具有交感效應。 |
英文摘要 | In vitro studieswere conducted to establish protocol for micropropagation of Rehmannia glutinosa Libosch, an important Chinese herbal medicine. Results showed that seeds disinfection with 0.6% sodium hypochlorite for 15 min significantly reduced the contamination rate to 5% and obtained the highest germination rate to 80%. Comparing the regeneration rate of leaf and petiole explants from in vitro grown seedlings revealed that petiole explants growing on MS medium containing 0.01 mg L^(-1) 1-Naphthaleneacetic acid with 2.0 mg L^(-1) Benzyladenine (BA) and cultivated in darkness obtained a better survival rate (62%) along with 10% shoot induction rate and 62% callus induction rate. Cultivating shoot tip and nodal stem segment of in vitro grow seedlings on the basal medium containing various sucrose concentrations (3- 9%) showed that addition of 3% sucrose had the highest survival rate (100%). Furthermore, shoot tip, inter-nodal and basal nodal stem segments of seedlings were cultured on the basal medium containing 3% sucrose and various BA concentrations (0.0- 1.0 mg L^(-1)) to evaluate their regenerative potential. The results showed that shoot tip had the highest survival rate (80- 100%) and had the most adventitious shoots induced per explant when cultured on the medium containing 0.5 mg L^(-1) BA. The results show that type of stem segment explants and BA concentration are key factors influence the micorpropagation of Rehmannia glutinosa Libosch. |
本系統中英文摘要資訊取自各篇刊載內容。