查詢結果分析
相關文獻
- Sweet Potato Storage Root Thioredoxin h2 and Their Peptic Hydrolysates Exhibited Angiotensin Converting Enzyme Inhibitory Activity in Vitro
- Sweet Potato Storage Root Defensin and Its Tryptic Hydrolysates Exhibited Angiotensin Converting Enzyme Inhibitory Activity in Vitro
- Sweet Potato (Ipomoea Batatas (L.)Lam. 'Tainong 57')Storage Root Mucilage Exhibited Angiotensin Converting Enzyme Inhibitory Activity in Vitro
- Sweet Potato Storage Root Thioredoxin h2 with both Dehydroascorbate Reductase and Monodehydroascorbate Reductase Activites
- Sweet Potato Storage Root Trypsin Inhibitor and Their Peptic Hydrolysates Exhibited Angiotensin Converting Enzyme Inhibitory Activity in Vitro
- 血管收縮素轉化酶抑制劑
- The Influence of Dust on Physiological Responses of Sweet Potato (Ipomoea Batatas) Leaves
- 甘藷收穫機之研製與改良
- 自乳清蛋白水解液中製備血管收縮素轉化酶抑制劑之研究
- 血管收縮素轉化酶抑制劑在糖尿病病人的使用
頁籤選單縮合
題 名 | Sweet Potato Storage Root Thioredoxin h2 and Their Peptic Hydrolysates Exhibited Angiotensin Converting Enzyme Inhibitory Activity in Vitro=甘藷塊根中硫氧化還原蛋白h2及其合成之胜肽具有血管收縮素轉化酶抑制活性 |
---|---|
作 者 | 黃冠中; 陳顯榮; 北中進; 吳金濱; 侯文琪; 吳介信; 許明志; 黃世勳; 林耀輝; | 書刊名 | Botanical Studies |
卷 期 | 52:1 2011.01[民100.01] |
頁 次 | 頁15-22 |
分類號 | 434.313 |
關鍵詞 | 甘藷; 血管收縮素轉化酶; 硫氧化還原蛋白h2; 水解胜肽; Angiotensin converting enzyme; ACE; Hydrolysis peptides; Sweet potato; Thioredoxin h2; |
語 文 | 英文(English) |
中文摘要 | 在大腸桿菌 (M15) 中大量表現重組蛋白質硫氧化還原蛋白 h2 (Trx h2),利用鎳離子螯合之親和性管柱純化。 Trx h2經 SDS-PAGE分析其分子量約為 14 kDa。由於 Trx h2具有抗氧化活性,去氫抗壞血酸還原酶,單去氫抗壞血酸還原酶的活性。 Trx h2 以 (N-(3-[2-furyl] acryloyl)-Phe-Gly-Gly) (FAPGG)為受質,利用分光光度計的方法分析抑制血管收縮素轉化酶 (angiotensin converting enzyme, ACE) 的能力,其效果隨劑量增加而增加 (50 到 200 µg/mL TI,分別抑制 31.9-65.9 % 血管收縮素轉化酶活性)。Trx h2對於血管收縮素轉化酶之 50% 抑制濃度 (IC50) 為 151.8 µg/mL,對照組 Captopril 為 10 nM (868 ng/mL)。另外利用螢光 silica TLC 偵測 FAPGG 及其水解產物 FAP,結果也顯示 Trx h2對於血管收縮素轉化酶有抑制的效果。 Trx h2對於血管收縮素轉化酶是屬於混合型抑制。而當加入 200 μg/mL Trx h2 時, Vmax和 Km分別為 0.010 ∆A/min 和 0.125 mM;而未入 Trx h2時, Vmax和 Km分別為 0.0096 ∆A/min和 0.495 mM利用胰蛋白酶以不同時間水解 Trx h2時,發現反應 16小時時其血管收縮素轉化酶活性有抑制的效果可以從 52 % (0 h) 增加到 72 % (16 h)。由結果可知小分子的胜肽會隨著水解時間增加且血管收縮素轉化酶活性抑制也有增加,但水解時間超過 16 h時,血管收縮素轉化酶活性抑制會降低,可能是由於一些胜肽的結構被破壞。利用電腦模擬胰蛋白酶水解 Trx h2的結果,四種人工合成具有抑制血管收縮素轉化酶活性胜肽: EVPK, VVGAK, FTDVDFIK 和 MMEPMVK,測定其 IC50為 1.73 ± 0.24, 1.14 ± 0.13, 0.42 ± 0.02 和 1.03 ± 0.58 mM。結果發現 FTDVDFIK 具有很好的抑制血管收縮素轉化酶活性。當人們食用甘藷塊根時,Trx h2及其胜肽也許對於高血壓和其他疾病的控制是有益的。 |
英文摘要 | Recombinant thioredoxin h (Trx h2) overproduced in E. coli (M15) was purified by Ni2+-chelate affinity chromatography as previously reported (Huang et al., 2004a). The molecular mass of Trx h2 was ca. 14 kDa as determined by SDS (sodium dodecyl sulfate)-PAGE (polyacrylamide gel electrophoresis). Trx h2 had antioxidant (Huang et al., 2004b), dehydroascorbate reductase, and monodehydroascorbate reductase activities (Huang et al., 2008a). Trx h2 was shown by spectrophotometric methods to inhibit angiotensin converting enzyme (ACE) in a dose-dependent manner (50-200 µg/mL, with 31.9 ~ 65.9% inhibition) using N-[3(2-furyl) acryloyl]-Phe-Gly-Gly (FAPGG) as a substrate. A 50% inhibition (IC50) of ACE activity required 151.8 μg/mL of Trx h2 and 10 nM (868 ng/mL) of Captopril. TLC data also showed Trx h2 as an ACE inhibitor. Trx h2 acted as a mixed type inhibitor against ACE using FAPGG as a substrate. When 200 μg/mL Trx h2 were added, Vmax and Km were, respectively, 0.010 ∆A/min and 0.125 mM; without Trx h2 they were 0.0096 ∆A/min and 0.495 mM. Trypsin was used for Trx h2 hydrolysis over different time periods. ACE inhibitory activity was found to increase from 52% to about 72% after 16 h of hydrolysis. The results suggested that the ACE inhibitory capacity of small peptides increased through trypsin hydrolysis of Trx h2 up to 16 h and then decreased, which may have been due to the disappearance of some active ingredients. Four peptides, namely EVPK, VVGAK, FTDVDFIK and MMEPMVK, were synthesized based on the simulated trypsin digestion of Trx h2 and then tested for ACE inhibitory activity. The IC50 values of individual peptides were 1.73 ± 0.24, 1.14 ± 0.13, 0.42 ± 0.02, and 1.03 ± 0.58 mM, respectively, suggesting that FTDVDFIK might be the main active site of ACE inhibition.The results for Trx h2 and its hydrolysates might mean that consumption of sweet potato storage roots can aid in the control of hypertension and other diseases. |
本系統中英文摘要資訊取自各篇刊載內容。