查詢結果分析
相關文獻
- 豬誘導多能性幹細胞移植追蹤之研究
- Germ Cell Tumor in Infancy and Childhood: Experience at One Institute Compared to Other Studies
- 原發性免疫不全疾病的造血幹細胞移植治療
- Allogeneic Stem Cell Transplantation for Patients with High-Risk Myelodysplastic Syndrome
- 造血細胞移植的新進展--非骨髓去除法移植的現況
- 造血幹細胞移植的演進
- 自體移植法治療白斑症--以表皮移植片合併培養的黑色素細胞移植治療白斑成功之病例
- Mature Teratoma with Secodary Infection: Case Report
- 以培養之純黑色素細胞移植治療分節型白斑--十九例治療結果分析
- 局部放射治療對腦幹部位不成熟畸胎瘤之治療效果--病例報告
頁籤選單縮合
題名 | 豬誘導多能性幹細胞移植追蹤之研究=Study of Traceability of Porcine Induced Pluripotent Stem Cells after Transplantation |
---|---|
作者姓名(中文) | 廖御靜; 劉宇旃; 陳怡秀; 李佳馨; 陳立人; 楊鎮榮; | 書刊名 | 畜產研究 |
卷期 | 48:3 2015.09[民104.09] |
頁次 | 頁196-203 |
分類號 | 437.21 |
關鍵詞 | 豬誘導多能性幹細胞; 綠色螢光蛋白質; 自體分化; 畸胎瘤; 細胞移植; Porcine induced pluripotent stem cells; Green fluorescent protein; Spontaneous differentiation; Teratoma; |
語文 | 中文(Chinese) |
中文摘要 | 研究目的為建立表現綠色螢光蛋白質之豬誘導多能性幹細胞(porcine induced pluripotent stem cell, piPS cell) 與探討細胞移植後之追蹤性。試驗係利用電穿孔法施以2 次直流電脈衝,將外源性綠色螢光蛋白質(green fluorescent protein, GFP) 報導基因轉染進畜試黑豬誘導多能性幹細胞,探討經轉染後之細胞的GFP 基因表現並檢視其多能性分化能力,以及其經活體移植後之表現。實驗結果顯示,piPS 細胞經轉染後可以持續且穩定表現綠色螢光;利用懸浮培養技術將細胞分化成類胚體與自體分化為三胚層後,綠色螢光仍可持續表現。此外,將表現綠色螢光之piPS 細胞移植於非肥胖糖尿病型重症聯合免疫缺陷型(nonobese diabetic/severe combined immunodeficiency, NOD-SCID) 小鼠,可誘發形成畸胎瘤(teratoma),且移植部位之螢光表現可於移植後第30 天利用活體影像分析系統檢出;畸胎瘤體積於piPS 細胞移植後90 天內顯著增加,綠色螢光強度亦逐次明顯。因此,本研究所建立之表現綠色螢光的piPS細胞不論是細胞分化與細胞移植後,GFP 之表現持續且穩定,可做為細胞移植後之活體追蹤與即時監控之標的,有利於細胞移植治療研究。 |
英文摘要 | The purpose of this study was to establish the green fluorescent protein expressing porcine induced pluripotent stem cells (piPS/GFP+ cells) and to investigate the traceability after cell transplantation. By using electroporation with 2 DC pluses of 150 V/cm and 10 mini sec, we successfully transfected GFP reporter gene into piPS cells, thereafter, which continuously and steadily expressed GFP signal. The embryoid formation derived from piPS/GFP+ cells by hanging drop culture also maintained GFP expression, induced and spontaneous differentiated into cells and tissues of the three germ layers. Furthermore, the teratomas were found in the NOD-SCID mice after piPS/GFP+ cells transplantation. The GFP expression of the transplanted piPS/GFP+ could be detected by using in vivo imaging system on day 30 after transplantation. The size of teratomas was significantly enlarged on day 90 after transplantation, along with the increment of the GFP signal intensity. Therefore, the GFP expression of piPS/GFP+ cells can be continuously and steadily maintained regardless of the differentiation or transplantation. The piPS/GFP+ cells could serve as a traceable target after transplantation and could be beneficial for the development of cell transplantation therapeutics. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。