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| 題 名 | Diversity of Fusarium Species Associated with Maize and Sorghum Grains Grown in Karnataka, India=印度卡那塔克邦地區玉米及高粱榖粒上鐮孢菌之多樣性 |
|---|---|
| 作 者 | M. Y. Sreenivasa; Adkar Purushothama Charithraj; Regina Sharmila Dass; G. R. Janardhana; | 書刊名 | 中華真菌學會會刊 |
| 卷 期 | 27:1 2012.07[民101.07] |
| 頁 次 | 頁17-29 |
| 分類號 | 379.1 |
| 關鍵詞 | 玉米; 多樣性; 高粱; 發生頻率; 鐮孢菌; Diversity; Incidence; Fusarium; ITS; Maize; Sorghum; |
| 語 文 | 英文(English) |
| 中文摘要 | 從卡那塔克邦之15個地區中採集了130個玉米及高粱樣本,進行鐮孢菌(Fusarium)多樣性及發生頻率之分析。結果可發現10種鐮孢菌存在於玉米及高粱之榖粒,為 F. verticillioides、F. proliferatum、F. oxysporum、F. anthophilum、F. pallidoroseum、F. sporotrichioides、F. solani、F. graminearum、F. decemcellulare及F. lateritium。在玉米上以F. verticillioides、F. proliferatum及F. anthophilum之發生頻率最高,分別為 18.69%、6.08%及4.46%;在高粱上以F. verticillioides及F. anthophilum之發生頻率最高,分別為10.22%及1.67%。此多樣性及發生頻率之調查結果,將有助於預測玉米及高粱榖粒採收後受到鐮孢菌影響所造成之儲藏性腐壞及其毒素之產生。 |
| 英文摘要 | A total of 130 maize and sorghum samples from 15 districts of Karnataka state were analyzed for diversity and per cent incidence of Fusarium species by direct plating method on malachite green agar 2.5 medium. For further identification of Fusarium species, one set of genus-specific ITS primers was used to differentiate Fusarium species from closely related genera by polymerase chain reaction. The study revealed the occurrence of 10 diverse species of Fusarium in maize and sorghum samples collected from different districts of Karnataka. The species identified were F. verticillioides, F. proliferatum, F. oxysporum, F. anthophilum, F. pallidoroseum, F. sporotrichioides, F. solani, F. graminearum, F. decemcellulare, and F. lateritium respectively. All the Fusarium species tested by PCR scored positive with the ITS pair of primers with an expected 431bp PCR product. The study further revealed the occurrence of F. verticillioides (18.69%), F. proliferatum (6.08%), and F. anthophilum (4.46%) in maize samples respectively. Similarly, species of F. verticillioides (10.22%) and F. anthophilum (1.67%) were recorded in higher per cent incidence in sorghum samples. The data on the diversity and incidence of Fusarium species would be of great significance for predicting the extent of post-harvest deterioration and subsequent accumulation of toxins in maize and sorghum grains produced in Karnataka, India. |
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