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| 題 名 | 臺灣草莓灰黴病菌(Botrytis cinerea)對Strobilurin類殺菌劑之抗藥性機制探討=Study of the Mechanism of Strobilurins Resistance in Botrytis Cinerea from Taiwan |
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| 作 者 | 陳麗淑; 鍾文全; 鍾文鑫; | 書刊名 | 農林學報 |
| 卷 期 | 59:3 2010.09[民99.09] |
| 頁 次 | 頁231-252 |
| 分類號 | 435.365 |
| 關鍵詞 | 草莓灰黴病菌; 抗藥性機制; Botrytis cinerea; QoI; Resistant mechanism; AOX; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 前人研究得知台灣草莓灰黴病菌 (Botrytis cinerea)抗 Strobilurin (QoI)類之機制與 cytochrome b (cyt b)基因之突變並無直接相關,為釐清台灣草莓灰黴病菌抗 QoI類藥劑之可能機制,本研究分別探討抗 QoI類灰黴病菌之粒腺體的改變和 alternative oxidase (AOX)是否與抗藥性產生有關。結果顯示經 100 μg/ml亞托敏原體處理後,抗性與感性兩菌株之細胞內粒腺體的數目與形態均無明顯差異。而在 AOX對抗藥性影響方面,於含 100 μg/ml亞托敏原體的 PDA培養基中加入可抑制 AOX活性之 salicylhydroxamic acid (SHAM)藥劑,並進行菌絲生長測試,得知抗藥性菌株之抗藥性明顯降低,而感受性菌株之菌絲生長抑制率為 100%。此結果顯示 alternative pathway respiration可能參與草莓灰黴病菌抗藥性產生之反應。此外測試灰黴病菌株之耗氧情形,證實孢子懸浮液添加 SHAM之後,感受性菌株的呼吸作用明顯受到抑制,而抗性菌株之呼吸作用受抑制程度隨供試菌株不同而有差異。進一步分析添加 100 μg/ml亞托敏原體後灰黴病菌菌株 AOX基因的表現,結果顯示所測試抗 QoI類藥劑之灰黴病菌菌株的 AOX基因表現存在差異性。 |
| 英文摘要 | Previous study indicated that the mechanism of isolates to strobilurins (QoIs) in Botrytis cinerea was not relative to mutation of cytochrome b (cyt b) gene. For carry out the mechanism of resistance, the morphology and number of mitochondria and role of alternative oxidase (AOX) in B. cinerea were analyzed. The results revealed that the variation of morphology and number on mitochondria particle was not signification between QoIs resistant isolates and sensitive isolates. Moreover, added salicylhydroxamic acid (SHAM), the AOX inhibitor, in PDA medium with 100 μg/ml azoxystrobin was significant to reduce the resistant level to QoIs in resistant and sensitive isolates of B. cinerea, especially, the inhibition rate of mycelial growth of sensitive isolates was 100%. The results demonstrated that alternative pathway respiration may affect the resistance of B. cinerea to QoIs. In addition, the effect of SHAM on inhibition of oxygen consuming of sensitive isolates of B. cinerea was obvious. However, the variation of oxygen consuming was observed among resistant isolates of B. cinerea. Thus, the respiration of resistant isolates of B. cinerea could not be affected by SHAM completely. Furthermore, the AOX gene expression analysis revealed the QoIs resistant isolates of B. cinerea were variable. |
本系統中英文摘要資訊取自各篇刊載內容。