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題名 | 利用形態性狀及RAPD、ISSR分子標誌探討馬祖東引地區換錦石蒜(Lycoris sprengeri)種原歧異度=Genetic Diversity of Lycoris sprengeri in Ma-Tzu Using Phenotype, RAPD and ISSR Molecular Markers |
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作者 | 蔣順惠; 葉志新; 蔡錫鍊; 曹爾元; 廖芳心; 傅仰人; | 書刊名 | 桃園區農業改良場研究彙報 |
卷期 | 72 2012.12[民101.12] |
頁次 | 頁27-44 |
分類號 | 435.453 |
關鍵詞 | 換錦石蒜; 東引; 種原歧異度; 逢機增幅多型性DNA; 簡單重複序列間多型性; Lycoris sprengeri; Tung-yin Township; Genetic diversity; RAPD; ISSR; |
語文 | 中文(Chinese) |
中文摘要 | 本研究針對分布於福建省連江縣(馬祖)東引鄉之換錦石蒜(Lycoris sprengeri)進行種原蒐集,並調查其形態特性及分析DNA分子標誌,探討東引島嶼忠靈堂峽灣及紫澳,與西引島后澳及東澳等四個棲地換錦石蒜族群間及族群內個體遺傳歧異度,以供復育繁殖及育種之參考。現地外觀性狀調查包括:花莖長、小花數、花瓣長、花瓣寬、總花徑、小花徑及花色等,其中花色依淺、中、深及極深分級。另於四個棲地分別對不同花色植株採樣,及相關種金花石蒜(L. aurea)共32個樣品,使用15個RAPD及6個ISSR分子標誌進行擴增,並以NT-SYS軟體分析繪出樹狀圖,以了解換錦花族群之親緣關係。性狀調查結果顯示,同花色不同棲地間族群花梗長、花瓣長及總花徑等項目表現存在明顯差異。21個分子標誌分析結果,整體相似度在0.64-0.85之間,各棲地族群內具歧異度,對應外觀上棲地內植株具有不同花色的現象,但四個棲地間換錦石蒜並無明顯地理區隔現象。此結果顯示各棲地內具多元的遺傳背景,有利於東引特有的換錦石蒜族群適應自然環境的變遷,此豐富遺傳資源除可應用於育種,更值得積極保育之。 |
英文摘要 | The purpose of this research was discussing genetic diversities within groups and between groups of four habitants of "Lycoris sprengeri" native in Tung-yin Township in Ma-tzu. Through collection of genetic resource and phenotype data of "Lycoris sprengeri" in Tung-yin, the relationship between individuals in different habitants was figured and useful for the following work of restoration and breeding. The investigation included morphological and molecular marker analysis. Phenotypes included length of scape and petal, width of petal, floret number, size of floret and cyme. In molecular marker analysis, DNA samples of "Lycoris sprengeri" collected from 4 habitats was analyzed with 15 RAPD markers and 6 ISSR markers. Through genetic similarity matrix and Unweighted Pair Group Method with Arithmetic Mean conducted by NT-SYS, the genetic diversity of "Lycoris sprengeri" in Tung-yin was estimated by similarity tree and principle coordinates analysis results. As a result, there were significant differences in length of scape, length of petal and diameter of cyme between individuals of the same color types in different habitants. On the other hand, the similarity coefficiency of the collected samples was ranged in 0.64-0.85, and there was no obvious grouping among individuals in the same habitats. However, diversities was existed in each habitats. The results benefited "Lycoris sprengeri" adapting to environmental changes and work of restoration and breeding program we want. It is worth to preserve the diversely genetic resources in the small island that could be considered as secondary center of diversity. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。