頁籤選單縮合
題 名 | Identification of Differentially Expressed Proteins in TCV-infected Nicotiana Benthamiana Protoplasts Determined by 2-D Gel Electrophoresis=以二維膠體電泳法鑑定蕪菁皺葉病毒(TCV)感染的圓葉菸草原生質體內差別表現的蛋白質 |
---|---|
作 者 | Gao, Ruimin; Xie, Juntao; Lin, Qingsong; Seow, Teck Keong; Wong, Sek-man; | 書刊名 | 植物病理學會刊 |
卷 期 | 23:1 2014.03[民103.03] |
頁 次 | 頁15-29 |
分類號 | 433.4 |
關鍵詞 | 二維膠體電泳法; 蕪菁皺葉病毒; 圓葉菸草; Differential protein expression; TCV; 2-DE; Protoplasts; Chloroplasts; |
語 文 | 英文(English) |
中文摘要 | 有鑑於蕪菁皺葉病毒(Turnip crinkle virus, TCV)感染植物的機制尚未完全明瞭,加以尚無應用蛋白質體學的技術於此領域的研究,本研究應用二維膠體電泳及質譜量測等技術以闡明被TCV感染的圓葉菸草(Nicotiana benthamiana)原生質體及葉綠體內差別表現的蛋白質。研究中發現14種蛋白質分別與光合作用及醣類代謝有關[如:ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), RuBisCO activase, chlorophyll a/b binding protein, ferredoxin-NADP reductase,和triose phosphate isomerase]、與逆境反應和解毒功能有關(如heat shock protein 70和ascorbate peroxidase)、與蛋白處理和分解有關(如:FKBP-type peptidyl-prolyl cis-trans isomerase和zinc protease)、與能量代謝有關(如:adenylate kinase和ATP synthase)、與維持酸鹼度有關(如:carbonic anhydrase)、以及與TCV的結構有關(如:TCV coat protein)。以此研究方法,吾人得以區別RuBisCO不同次單位的表現量之差異,也可以區別葉綠素a/b鍵結蛋白的不同異構型。再者,本文首次揭露多種與植物病毒感染過程相關的差別表現的蛋白,例如FKBP和一種推定的鋅蛋白酶。 |
英文摘要 | As the mechanism of Turnip crinkle virus (TCV) infection in plants is still not fully understood and that there are no known studies on this using the proteomic approach, two-dimensional gel electrophoresis and mass spectrometry were used to elucidate the differentially expressed proteins in the protoplasts and chloroplasts of mock-inoculated and TCV-infected Nicotiana benthamiana leaves. The fourteen differentially expressed proteins were proteins associated with photosynthesis and carbohydrate metabolism [ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), RuBisCO activase, chlorophyll a/b binding protein, ferredoxin-NADP reductase, and triose phosphate isomerase], stress response and detoxification (heat shock protein 70 and ascorbate peroxidase), protein processing and degradation (FKBP-type peptidyl-prolyl cis-trans isomerase and zinc protease), energy metabolism (adenylate kinase and ATP synthase), pH maintenance (carbonic anhydrase), and TCV structure (TCV coat protein). Using this approach, we were able to distinguish the differing expression levels of different subunits of RuBisCO and different isoforms of chlorophyll a/b-binding proteins. Furthermore, for the first time, several of the differentially expressed proteins identified such as FKBP and a putative zinc protease were found to be associated with plant virus infection. |
本系統中英文摘要資訊取自各篇刊載內容。