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頁籤選單縮合
題名 | ELISA Analysis of Serum VEGF in Oral Squamous Cell Carcinoma Patients=口腔癌患者血清血管內皮生長因子之表現與研究分析 |
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作者 | 鄭介原; 劉崇基; | 書刊名 | 臺灣口腔顎面外科學會雜誌 |
卷期 | 25:4 2014.12[民103.12] |
頁次 | 頁233-239 |
分類號 | 416.89 |
關鍵詞 | 血管內皮生長因子; 多型性變化; 酵素免疫分析法; 口腔鱗狀細胞癌; VEGF; Polymorphisms; ELISA; OSCC; |
語文 | 英文(English) |
中文摘要 | 本實驗的研究目的是針對口腔癌患者的血清血管內皮生長因子(VEGF)濃度與血液VEGF 936 CT多型性變化分析做相關的探討。根據之前的研究發現,口腔癌患者血液VEGF 936 CT多型性變化與其血管侵犯有明顯的相關,VEGF 936 C對偶基因是否藉由血清VEGF濃度的上升來造成血管侵犯的差異,而其臨床上所表現的差異也將有所探討。研究方法:本研究共搜集61位口腔癌患者,5位為前病變患者及7位正常對照組血液及臨床資料,VEGF 936 CT多型性變化利用聚合海連鎖反應及限制性片段長度多態性技術做分析,而VEGF血清濃度則以酵素免疫分析法三重複做定量,所有資料再以P4統計軟體分析。結果:腫瘤大小(T1,T2,T3)及分化差之癌症患者其血清中VEGF濃度相對量也明顯增加(p=0.01)。而VEGF 936 CC多型性口腔癌患者及血管侵犯患者其血清中VEGF濃度也比VEGF 936 CT及VEGF 936 TT患者來得高(p=0.04)。結論:VEGF 936 CT多型性變化在腫瘤血管侵犯上扮演重要角色,而血清中VEGF濃度的增加亦呈現正相關,我們或許可以從中做更進一步的研究來釐清相關的機轉。 |
英文摘要 | The present study was aimed to investigate both serum vascular endothelial growth factor (VEGF) concentrations and VEGF 936 CT polymorphisms in patients with oral squamous cell carcinoma (OSCC). We also clarify whether VEGF 936 C allele is associated with vascular invasion in OSCC via up regulation of VEGF circulating levels in OSCC patients. The study consisted of 61 patients with OSCC, 5 precancerous patients and 7 normal individuals. VEGF 936 CIT polymorphisms were determined by using polymerase chain reaction and restriction fragment length polymorphism. Concentrations of serum VEGF was determined by commercial enzyme-linked immunosorbent assay (ELISA) kits. These data demonstrate that tumor size T1, T2, T3 and poor differentiation pattern of OSCC patients have significant higher serum level of VEGF (p=0.01).The VEGF 936 C/C polymorphism and vascular invasion of cancerous patients also have significant higher VEGF serum level than those with CIT, TIT polymorphism and without vascular invasion. (p=0.04). |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。