查詢結果分析
來源資料
頁籤選單縮合
題 名 | 蛹蟲草發酵液凍乾粉之基因毒性及急毒性分析=Genotoxicity Test and Acute Oral Toxicity Test of Cordyceps Militaris Mycelium |
---|---|
作 者 | 劉曉卉; 謝逸璇; 陳家榮; 施君翰; 彭依婷; 陳勁初; | 書刊名 | 檢驗及品保雜誌 |
卷 期 | 4:1 2015.02[民104.02] |
頁 次 | 頁23-33 |
分類號 | 368.71 |
關鍵詞 | 蛹蟲草發酵液凍乾粉; 口服急性毒性試驗; 基因毒性試驗; Freeze-dried Cordyceps militaris mycelium powder; Oral acute toxicity; Genotoxicity test; |
語 文 | 中文(Chinese) |
中文摘要 | 蛹蟲草(Cordyceps militaris)又稱為北冬蟲夏草,是目前研究最多的蟲草之一。目前已有美、中、日、台及韓等國工業化液態發酵量產且產品上市,但相關安全性研究文獻稀少,本研究利用液態發酵技術培養蛹蟲草菌絲體,並針對蛹蟲草菌絲發酵液凍乾粉進行三項基因毒性試驗以及單一劑量口服急性毒性試驗,評估蛹蟲草菌絲發酵液凍乾粉之毒性。在沙門氏菌回復突變試驗中,測試樣品以無菌水配置50 mg/mL,5.0 mg/plate為最高測試劑量,再以無菌水序列稀釋至0.3125 mg/plate等劑量。無論是否經過S9mix處理,對Salmonella typhimurium TA97a、TA98、TA100、TA102及TA1535菌株之回復突變菌落數,與負對照組相較,均未達陽性判定標準。在體外哺乳類細胞染色體結構異常試驗中,以哺乳類細胞CHO-K1是否具誘導染色體變異的能力來評估基因毒性,在不含S9下作用3小時、20小時以及含有S9下作用3小時之三種方式處理下,均未造成細胞5 %以上染色體差異,且與負對照組之間無顯著差異,以最高濃度5.0 mg/mL處理下,仍未超過50 %細胞死亡之細胞毒性,因此,判定試驗結果為陰性反應。另外,在囓齒類動物體內週邊血液微核試驗中,以口服途徑餵食ICR小鼠,劑量分別為1,000、2,000 及3,000 mg/kg b.w.,測試劑量之網狀紅血球的比例(reticulocyte, RET %)及微核率(micronucleus frequency, MN(per mille)RET)均與負對照組無顯著差異。在口服急性毒性試驗中,SD大鼠經口餵食單一高劑量2000 mg/kg b.w.後,活動狀況正常。在7天試驗期間,體重增長百分率(%)、臨床症狀及解剖肉眼檢查與對照組皆無差異。 |
英文摘要 | Cordyceps militaris, also known as north Chinese caterpillar fungus, is currently one of the most studies of Cordyceps. Though liquid fermentation for the industrial production has been proven successful to market in the United States, China, Japan, Taiwan, South Korea and other countries, there are few documents of genotoxicity safety researches. In this study, we focus on the three genotoxicity safety tests and single dose oral acute toxicity of freezedried Cordyceps militaris mycelium powder which is produced by liquid fermentation technology to define its toxicity. In vitro bacterial testing method of gene mutation, test samples were prepared as 50 mg/mL stock solution with sterile water and sequence from 5.0 mg/plate (highest testing dosage) to 0.3125 mg/plate. It' s shown no significant differences between treatment and the negative control group when we tested with Salmonella typhimurium TA97a, TA98, TA100, TA102 and TA1535 strains with or without metabolic activation system (S9 mixtu re). In vitro chromosomal aberration test is to identify the incidence of structural chromosomal aberrations ability in cultured mammalian cells (CHO-K1). The results shown less than 5 % chromosome mutated in the three metabolic systems (3 hrs w/ S9, 3 hrs w/o S9 and 20 hrs w/o S9) and no significant difference with control group. Even the highest concentration of 5.0 mg / mL treatment, there are more than 50 % of CHO-K1 cells remain. In vivo micronucleus testing of rodent peripheral blood, we studied the ICR mice and monitored their reticulocyte proportion (RET %) and micro-nuclear frequency (MN(per mille)RET) by treating different oral dosage with 1000, 2000 and 3,000 mg/kg (b.w.) The result has shown no difference between treatment and control group. Acute oral toxicity study, after oral feeding a single high dose of 2,000 mg / kg (b.w.) in SD rats, rats activity status is normal. The results showed that there is no difference between the control group on body weight gains (%)、clinical symptoms and observatio ns on the gross necropsy during continuous observation of seven days. |
本系統中英文摘要資訊取自各篇刊載內容。